Advances in Multiple Reaction Monitoring (MRM) for Targeted Proteomics in Biomedical Research.

Elyssa N Baker, Timon Geib, Christoph H Borchers

Abstract readReview

In one paragraph

Review in Advances in experimental medicine and biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Elyssa N BakerLady Davis Institute, McGill University, Montreal, QC, Canada.

Timon GeibLady Davis Institute, McGill University, Montreal, QC, Canada.

Christoph H BorchersLady Davis Institute, McGill University, Montreal, QC, Canada.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Targeted proteomics has emerged as a cornerstone of quantitative biomedical research, enabling precise, reproducible measurement of predefined proteins across complex biological matrices. Among targeted mass spectrometry approaches, multiple reaction monitoring (MRM), typically performed on triple quadrupole (QQQ) instruments, remains the gold standard for sensitive and accurate protein quantitation. Over the past decade, substantial advances in instrumentation, experimental workflows, and computational tools have significantly expanded the analytical depth, throughput, and accessibility of MRM-based assays. Concurrently, innovative strategies for proteome-wide quantitation are addressing longstanding challenges related to standardization, cost, and scalability. This chapter provides a comprehensive overview of the principles underlying MRM, recent technological and methodological advancements, and emerging applications in biomedical research, with an emphasis on biomarker validation, clinical proteomics, and absolute proteome profiling.

Indexed as

Biomedical ResearchMass SpectrometryProteomeProteomicsAnimalsBiomarkersHumansIsotope LabelingBiomarkersProteomeAbsolute quantitationClinical proteomicsMultiple reaction monitoringStable isotope-labeled standardsTargeted proteomicsTriple quadrupole mass spectrometry

Identifiers

PMID42907654

DOI10.1016/j.crmeth.2021.100015

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.