Evidence map›Paper›PMID 42905739›Full record

ArticlePloS one2026

Nomenclature, classification, and in-silico structural analysis of MSP1a variants of Anaplasma marginale.

Amam Zonaed Siddiki, Nazifa Humaira, Nanziba Sharmin Hossain, Yusra Bint Akbar, Puja Palit, Pann Ei Phwe, Rahima Akter Eva, Hala Alshaar, Marjia Islam Tia, Kethain Marma and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Amam Zonaed SiddikiGenomics Research Group, Department of Pathology and Parasitology, Chittagong Veterinary and Animal Sciences University (CVASU), Chittagong, Bangladesh.
Nazifa HumairaDepartment of Bioinformatics and Biotechnology, Asian University of Women (AUW), Chittagong, Bangladesh.ORCID https://orcid.org/0009-0003-2141-7956
Nanziba Sharmin HossainDepartment of Bioinformatics and Biotechnology, Asian University of Women (AUW), Chittagong, Bangladesh.ORCID https://orcid.org/0009-0001-1973-136X
Yusra Bint AkbarDepartment of Bioinformatics and Biotechnology, Asian University of Women (AUW), Chittagong, Bangladesh.ORCID https://orcid.org/0009-0009-0121-6384
Puja PalitDepartment of Bioinformatics and Biotechnology, Asian University of Women (AUW), Chittagong, Bangladesh.ORCID https://orcid.org/0009-0005-6486-6692
Pann Ei PhweDepartment of Bioinformatics and Biotechnology, Asian University of Women (AUW), Chittagong, Bangladesh.
Rahima Akter EvaDepartment of Bioinformatics and Biotechnology, Asian University of Women (AUW), Chittagong, Bangladesh.ORCID https://orcid.org/0009-0005-4332-6478
Hala AlshaarDepartment of Bioinformatics and Biotechnology, Asian University of Women (AUW), Chittagong, Bangladesh.
Marjia Islam TiaDepartment of Bioinformatics and Biotechnology, Asian University of Women (AUW), Chittagong, Bangladesh.ORCID https://orcid.org/0009-0005-5038-0771
Kethain MarmaDepartment of Bioinformatics and Biotechnology, Asian University of Women (AUW), Chittagong, Bangladesh.ORCID https://orcid.org/0009-0002-2940-3375
Barsha AkterDepartment of Bioinformatics and Biotechnology, Asian University of Women (AUW), Chittagong, Bangladesh.
Samira SultanaDepartment of Bioinformatics and Biotechnology, Asian University of Women (AUW), Chittagong, Bangladesh.ORCID https://orcid.org/0009-0000-2128-512X
Sabreena AlamGenomics Research Group, Department of Pathology and Parasitology, Chittagong Veterinary and Animal Sciences University (CVASU), Chittagong, Bangladesh.ORCID https://orcid.org/0000-0001-6058-4253

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bovine anaplasmosis, caused by the tick-borne rickettsial pathogen Anaplasma marginale, is a significant threat to livestock health and productivity. The major surface protein 1a (MSP1a) is a widely used molecular marker for characterizing strain diversity, due to its variable N-terminal tandem repeats. In this study, we analyzed and updated the nomenclature classification of 157 A. marginale MSP1a sequences submitted to NCBI between 2013 and 2023, following the research by Cabezas-Cruz in 2013. We identified and classified 189 unique tandem repeats, reported their tick-transmissibility, predicted their secondary structures and B-cell epitopes, and analyzed amino acid variability. Following this, we conducted a comparative analysis with previously published datasets, which revealed new tandem repeat types, new positions of serine within tandem repeats, and high variation among B-cell epitope variants. We have also classified the unique R1 tandem repeats of the new MSP1a sequences into different ecoregion clusters based on the principal component analysis of NDVI values. This updated classification system enhances the molecular understanding of A. marginale diversity and may contribute to future developments in strain typing, epidemiological investigations, and vaccine research.

Indexed as

Anaplasma marginaleMerozoite Surface Protein 1Amino Acid SequenceAnaplasmosisAnimalsBacterial Outer Membrane ProteinsCattleComputer SimulationEpitopes, B-LymphocyteGenetic VariationPhylogenyProtein Structure, SecondaryTandem Repeat SequencesTerminology as TopicBacterial Outer Membrane ProteinsEpitopes, B-Lymphocytemajor surface protein 1a, AnaplasmaMerozoite Surface Protein 1

Identifiers

PMID42905739
PMCPMC13686480

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.