Evidence map›Paper›PMID 42903866›Full record

ArticlehLife2026

Genetic tool for specific tracking of mature neutrophils.

Jiayu Cao, Hui Ping Yaw, Shilin Yi, Yanxi Zhou, Shifan Qin, Yuanyuan Wang, Raul da Costa, Lei Zhang, Dandan Wu, Changbin Chen and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in hLife, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Jiayu CaoDepartment of Neurosurgery, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Hui Ping YawShanghai Immune Therapy Institute, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Shilin YiDepartment of Neurosurgery, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Yanxi ZhouShanghai Immune Therapy Institute, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Shifan QinShanghai Immune Therapy Institute, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Yuanyuan WangJoint Laboratory for Biomedical Research and Pharmaceutical Innovation, The Unit of Pathogenic Fungal Infection & Host Immunity, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China.
Raul da CostaSingapore Immunology Network (SIgN), Agency for Science, Technology and Research (A∗STAR), Singapore, Singapore.
Lei ZhangShanghai Immune Therapy Institute, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Dandan WuShanghai Immune Therapy Institute, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Changbin ChenJoint Laboratory for Biomedical Research and Pharmaceutical Innovation, The Unit of Pathogenic Fungal Infection & Host Immunity, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China.
Melissa NgSingapore Immunology Network (SIgN), Agency for Science, Technology and Research (A∗STAR), Singapore, Singapore.
Immanuel KwokSingapore Immunology Network (SIgN), Agency for Science, Technology and Research (A∗STAR), Singapore, Singapore.
Oliver SoehnleinInstitute of Experimental Pathology, Center for Molecular Biology of Inflammation, University Hospital Münster, University of Münster, Münster, Germany.
Xiaoxiang ChenDepartment of Allergy, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Jieqing WanDepartment of Neurosurgery, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Lai Guan NgShanghai Immune Therapy Institute, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Tracking mature neutrophils remains challenging due to the lack of reliable cell surface markers. Although CD101 is a promising candidate as a marker for mature neutrophils, its stability under pathological conditions is unclear. Here, we developed a novel CD101-tdTomato reporter mouse model to specifically identify mature neutrophils. CD101 expression, tdTomato fluorescence, and neutrophil physiology were characterized by flow cytometry across peripheral tissues under homeostasis and by a transwell migration assay, in parallel with morphological features assessed by Wright-Giemsa staining. To assess marker stability, CD101-tdTomato and wild-type (WT) mice were treated with lipopolysaccharide (LPS) (200 ng, intraperitoneally, 2 h,

Indexed as

CD101immature neutrophilslineage tracingmature neutrophils

Identifiers

PMID42903866
PMCPMC13681518

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.