Evidence map›Paper›PMID 42902360›Full record

ArticleMicrobial cell factories2026

Identification, functional characterization of a novel flavohemoglobin from Ensifer adhaerens and its application to enhancing vitamin B

Jinmei Zhu, Shiyu Wang, Bo Li, Bolun Wang, Ping Wang, Jiequn Wu, Zejian Wang

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Article in Microbial cell factories, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Jinmei ZhuCollaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou, 310014, China.
Shiyu WangCollaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou, 310014, China.
Bo LiState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
Bolun WangCollaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou, 310014, China.
Ping WangState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
Jiequn WuCollaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou, 310014, China. jiequnwu@zjut.edu.cn.
Zejian WangState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China. wangzejian@ecust.edu.cn.

Funding

2025 Shanghai Key Technology R&D Program for Synthetic Biology 25HC2820400National Science and Technology Major Project of China 2025ZD1203400the "Challenge System" for County-level Specialized Industry Science and Technology Projects in Shijiazhuang City 247790652A
6 · The paper itself

Abstract

backgroundBacterial flavohemoglobins are well-characterized as versatile proteins that significantly augment the biosynthesis yields of diverse value-added compounds in engineered microbial platforms. Our previous studies revealed marked upregulation of a flavohemoglobin-encoding gene in Ensifer adhaerens under oxygen-limiting conditions, with potential associations to vitamin B

resultsIn this study, a novel flavohemoglobin (FHP, encoded by b12fla gene) from Ensifer adhaerens were characterized. FHP exhibited high sequence and structural similarity to HMP (the flavohaemoglobin from Halalkalibacterium halodurans), yet demonstrating 3-fold higher NADH oxidase activity. Heterologous expression of FHP in Escherichia coli positively influences cell growth, biomass yield on carbon and nitrogen sources, increased OD

conclusionsA flavohemoglobin from a new source was identified in this study. Heterologous expression in E. coli validated its ability to promote cell growth under specific oxygen supply conditions. Overexpression in E. adhaerens significantly enhanced cell growth and cell metabolism, while markedly increasing vitamin B

Indexed as

Bacterial ProteinsHemeproteinsVitamin B 12Escherichia coliFermentationOxygenBacterial Proteinsflavohemoprotein, BacteriaHemeproteinsOxygenVitamin B 12Cell growthEnsifer adhaerensFlavohemoglobinVitamin B12

Identifiers

PMID42902360
PMCPMC13685156

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.