Evidence map›Paper›PMID 42902125›Full record

ArticleBMC plant biology2026

Genetic diversity assessment of edible-podded peas (Pisum sativum var. saccharatum and Pisum sativum var. macrocarpon) using morphological traits and SSR markers.

Haritika Sharma, Rajinder Kumar Dhall, Hanish Sharma, Neha Verma, Saurabh Yadav, Priti Sharma, Neha Rana, Manpreet Kaur

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Article in BMC plant biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

8 authors.

Haritika SharmaDepartment of Vegetable Science, College of Horticulture and Forestry, Punjab Agricultural University, Ludhiana, Punjab, India.ORCID http://orcid.org/0009-0003-1196-0533
Rajinder Kumar DhallDepartment of Vegetable Science, College of Horticulture and Forestry, Punjab Agricultural University, Ludhiana, Punjab, India. rajinderkumar@pau.edu.ORCID http://orcid.org/0000-0003-3055-9780
Hanish SharmaDepartment of Vegetable Science, College of Horticulture and Forestry, Punjab Agricultural University, Ludhiana, Punjab, India.ORCID http://orcid.org/0009-0007-1431-8430
Neha VermaGSK Institute of Genetics, Plant Breeding and Biotechnology, Punjab Agricultural University, Ludhiana, Punjab, India.
Saurabh YadavGSK Institute of Genetics, Plant Breeding and Biotechnology, Punjab Agricultural University, Ludhiana, Punjab, India.ORCID http://orcid.org/0009-0001-4651-6710
Priti SharmaSchool of Agricultural Biotechnology, College of Agriculture, Punjab Agricultural University, Ludhiana, Punjab, India.ORCID http://orcid.org/0000-0002-2878-2249
Neha RanaDepartment of Vegetable Science, College of Horticulture and Forestry, Punjab Agricultural University, Ludhiana, Punjab, India.ORCID http://orcid.org/0000-0001-6197-0578
Manpreet KaurGSK Institute of Genetics, Plant Breeding and Biotechnology, Punjab Agricultural University, Ludhiana, Punjab, India.ORCID http://orcid.org/0000-0001-6356-2092

Funding

Ministry of Science and Technology, Department of Biotechnology, Government of India BT/Ag/CoE/PAU-GSKIG/2020-21
6 · The paper itself

Abstract

backgroundEdible-podded peas viz., snow peas and sugar snap peas are valued for their tender edible pods, nutritional quality, export potential and increasing market demand. However, limited information is available regarding their genetic diversity and population structure. Therefore, the present study was undertaken to assess agro-morphological and molecular diversity among 25 edible-podded and two garden pea genotypes under field conditions during 2022-2024. Genetic diversity was evaluated using 27 phenotypic traits comprising 14 qualitative and 13 quantitative descriptors based on DUS guidelines, along with 40 SSR markers through Shannon Diversity Index, Mahalanobis D² statistics, principal component analysis, cluster analysis, AMOVA and STRUCTURE analysis.

resultsAmong qualitative traits, pod curvature showed highest variability (Shannon Diversity Index, H = 1.25) followed by seed colour (H = 1.21). Mahalanobis D² analysis grouped genotypes into 5 clusters, with highest inter-cluster distance (2061.31) among Cluster III (PED-18-1, PED-18-7, PED-21-6, Arka Sampoorna) and Cluster V (Him Palam Mithi Phali-2, Honey Snap, PED-21-2, Oregon Sugar Pod, PED-21-3, Royal Snow, Tarvedo Sugar), indicating considerable genetic divergence. Out of 40 SSR markers screened, 16 were polymorphic and generated 2-6 alleles per locus with moderate to high polymorphic information content of 0.50, while primer AA332 exhibited the highest PIC value (0.77). SSR-based clustering grouped the genotypes into two major clusters with distinct sub-clustering patterns. AMOVA revealed 91% of the genetic variation existed within populations and STRUCTURE analysis identified four subpopulations (K = 4), indicating moderate genetic heterogeneity among the genotypes.

conclusionsBased on the combined agro-morphological and SSR marker analyses, PED-18-1, PED-18-7, PED-21-6, Arka Sampoorna, and PED-21-2 emerged as the most distinct genotypes and represent valuable parental resources for edible-podded pea improvement. These genotypes can be utilized to enhance productivity, strengthen food and nutritional security and promote sustainabe breeding in pea growing regions.

Indexed as

Genetic VariationMicrosatellite RepeatsPisum sativumCluster AnalysisFruitGenetic MarkersGenotypePhenotypeGenetic MarkersEdible-podded peasGenetic diversityMahalanobis D² analysisMorphological traitsPopulation structureSSR markers

Identifiers

PMID42902125
PMCPMC13685107

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.