Evidence map›Paper›PMID 42901925›Full record

ArticleNature communications2026

Single-oocyte proteome-transcriptome co-profiling reveals a role of dysregulated lactate metabolism in oocyte aging.

Lanrui Cao, Hao Wu, Yirong Jiang, Yaxue Hou, Zhuo Yang, Yuan Wang, Peipei Ren, Panpan Zhao, Xiaofeng Ni, Shuai Liu and 11 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

21 authors.

Lanrui Cao *First Affiliated Hospital, Zhejiang University School of Medicine, and Liangzhu Laboratory of Zhejiang University, Hangzhou, China.
Hao Wu *First Affiliated Hospital, Zhejiang University School of Medicine, and Liangzhu Laboratory of Zhejiang University, Hangzhou, China.
Yirong Jiang *First Affiliated Hospital, Zhejiang University School of Medicine, and Liangzhu Laboratory of Zhejiang University, Hangzhou, China.ORCID 0000-0001-7070-4909
Yaxue Hou *First Affiliated Hospital, Zhejiang University School of Medicine, and Liangzhu Laboratory of Zhejiang University, Hangzhou, China.
Zhuo YangSchool of Pharmacy, Shenyang Pharmaceutical University, Shenyang, China.
Yuan WangZhejiang Provincial Key Laboratory for Cancer Molecular Cell Biology, Life Sciences Institute, Zhejiang University, Hangzhou, China.
Peipei RenAssisted Reproduction Unit, Department of Obstetrics and Gynecology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
Panpan ZhaoAssisted Reproduction Unit, Department of Obstetrics and Gynecology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
Xiaofeng NiInstitute of Microanalytical Systems, Department of Chemistry, Key Laboratory of Excited-State Materials of Zhejiang Province, Zhejiang University, Hangzhou, China.
Shuai LiuLiangzhu Laboratory of Zhejiang University, Hangzhou, China.ORCID 0000-0002-8405-6743
Na KongLiangzhu Laboratory of Zhejiang University, Hangzhou, China.
Xin ShengLiangzhu Laboratory of Zhejiang University, Hangzhou, China.ORCID 0000-0002-3620-3577
Yinli ZhangAssisted Reproduction Unit, Department of Obstetrics and Gynecology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, China.ORCID 0000-0001-8353-9615
Songying ZhangAssisted Reproduction Unit, Department of Obstetrics and Gynecology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, China.ORCID 0000-0001-8615-7196
Jianfeng XiaoDepartment of Reproductive Center, The First Affiliated Hospital of Shantou University Medical College, Shantou University, Shantou, China.
Cunqi YeZhejiang Provincial Key Laboratory for Cancer Molecular Cell Biology, Life Sciences Institute, Zhejiang University, Hangzhou, China.ORCID 0000-0003-3117-8680
Heng-Yu FanAssisted Reproduction Unit, Department of Obstetrics and Gynecology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, China.ORCID 0000-0003-4544-4724
Yongcheng WangFirst Affiliated Hospital, Zhejiang University School of Medicine, and Liangzhu Laboratory of Zhejiang University, Hangzhou, China. yongcheng@zju.edu.cn.ORCID 0000-0003-2820-4243
Xiaomei TongAssisted Reproduction Unit, Department of Obstetrics and Gynecology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, China. 3406028@zju.edu.cn.ORCID 0000-0002-6982-445X
Qun FangInstitute of Microanalytical Systems, Department of Chemistry, Key Laboratory of Excited-State Materials of Zhejiang Province, Zhejiang University, Hangzhou, China. fangqun@zju.edu.cn.ORCID 0000-0002-6250-252X
Xudong FuFirst Affiliated Hospital, Zhejiang University School of Medicine, and Liangzhu Laboratory of Zhejiang University, Hangzhou, China. xudongfu@zju.edu.cn.ORCID 0000-0001-7024-0200

Funding

National Natural Science Foundation of China (National Science Foundation of China) 32470840,32270852
6 · The paper itself

Abstract

The age-related decline in oocyte quality is a major risk factor for female infertility. Although transcriptome has been examined in aged oocytes, proteomic landscape, which reflects primary gene functional executors, remains largely unexplored. This gap limits our understanding of oocyte aging. To address this, we perform single-cell proteome/transcriptome co-profiling of GV/MII-aged oocytes from mice and humans, revealing species- and stage-specific proteomic/transcriptomic changes during oocyte aging. Strikingly, we observe uncoupled proteomic and transcriptomic alterations, indicating that proteomic changes in aged oocytes are not primarily driven by RNA levels. Furthermore, we capture molecular heterogeneity in aged oocytes and identify MCT4 as a candidate marker of oocyte aging. Functional studies suggest MCT4 contributes to aging-associated oocyte defects via lactate export, and its inhibition improves aged-oocyte quality. These findings indicate altered lactate metabolism as a candidate marker and intervention target for oocyte aging and underscore the value of our profiling in dissecting oocyte aging.

Indexed as

Cellular SenescenceLactic AcidOocytesProteomeTranscriptomeAnimalsFemaleGene Expression ProfilingHumansMiceMonocarboxylic Acid TransportersProteomicsSingle-Cell AnalysisLactic AcidMonocarboxylic Acid TransportersProteome

Identifiers

PMID42901925
PMCPMC13683357

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.