Evidence map›Paper›PMID 42898874›Full record

ReviewFrontiers in plant science2026

Live spatial validation of plant-microbe interactions in the rhizosphere with light-sheet fluorescence microscopy.

Xingshui Dong, Qilu Cheng, Junwei Ma, Feng Wang, Hui Lin

Abstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in Frontiers in plant science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Xingshui DongState Key Laboratory for Quality and Safety of Agro-Products, Zhejiang Provincial Key Laboratory of Agricultural Microbiomics, Institute of Environment, Resource, Soil and Fertilizer, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
Qilu ChengState Key Laboratory for Quality and Safety of Agro-Products, Zhejiang Provincial Key Laboratory of Agricultural Microbiomics, Institute of Environment, Resource, Soil and Fertilizer, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
Junwei MaState Key Laboratory for Quality and Safety of Agro-Products, Zhejiang Provincial Key Laboratory of Agricultural Microbiomics, Institute of Environment, Resource, Soil and Fertilizer, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
Feng WangState Key Laboratory for Quality and Safety of Agro-Products, Zhejiang Provincial Key Laboratory of Agricultural Microbiomics, Institute of Environment, Resource, Soil and Fertilizer, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
Hui LinState Key Laboratory for Quality and Safety of Agro-Products, Zhejiang Provincial Key Laboratory of Agricultural Microbiomics, Institute of Environment, Resource, Soil and Fertilizer, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Plant-microbe interactions in the rhizosphere are spatial events, yet mechanisms are often inferred from bulk samples, endpoint images, or community profiles averaged across heterogeneous root zones. Rather than surveying LSFM hardware or rhizosphere imaging broadly, this review evaluates light-sheet fluorescence microscopy (LSFM) as a platform for claim-level live spatial validation. Evidence is separated into four levels: A, direct LSFM evidence; B, an LSFM-compatible sample or device demonstrated with another modality; C, a correlative or validation modality; and D, a proposed future application. Within the verified full-text evidence set, direct evidence is concentrated in whole-rhizosphere tracking of Bacillus populations, plant reporter responses during early root-fungus interactions, and rhizobial attachment and infection of root hairs. Many arbuscular mycorrhizal and biocontrol applications remain prospective. We compare instrument and sample choices across field of view, numerical aperture, voxel sampling, time interval, spectral channels, and matrix realism; define root-centric metrics for arrival, residence, occupancy, growth, and persistence; and distinguish simultaneous, sequential

Indexed as

correlative microscopylight-sheet fluorescence microscopylive imagingplant-microbe interactionsrhizospherespatial validationtransparent soil

Identifiers

PMID42898874
PMCPMC13679359

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.