ArticleMethodsX2026
A culturomics-inspired multi-medium workflow for soil fungal isolation and ITS metabarcoding comparison.
Article in MethodsX, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This method article describes a reproducible workflow for recovering, identifying, and comparing soil-associated fungi using complementary culture-dependent and sequence-based approaches. The workflow was designed for environmental mycology studies in which fungal DNA detection needs to be complemented by the recovery of living isolates. It combines standardized soil suspension processing, parallel plating on five culture media, daily morphotype selection and purification, MALDI-TOF MS screening, targeted multilocus sequencing when required, and comparison with ITS1/ITS2 metabarcoding profiles. In its application to soil samples, the workflow recovered a diverse viable isolate collection, supporting its usefulness for expanding fungal recovery, improving lower-level taxonomic anchoring, enriching identification resources, and distinguishing culture-recovered from sequence-detected fungal fractions.•Five culture media are used in parallel to broaden the spectrum of viable soil fungi recovered under standardized incubation conditions.•Recovered isolates are identified using MALDI-TOF MS and targeted multilocus sequencing when necessary.•Culture-recovered taxa are compared with ITS1/ITS2 metabarcoding profiles to distinguish shared and method-specific fungal fractions.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.