Evidence map›Paper›PMID 42897654›Full record

ArticleBiology direct2026

Single-cell RNA sequencing reveals that TAF7 promotes colorectal cancer stemness by targeting SOX2.

Xiaohui Ai, Yupei Jiang, Xiaoqing Huang, Haitao Ning, Shaojun Wang, Fang Li, Shulin Wu, Jun Mo

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biology direct, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Xiaohui AiDepartment of Tumor Surgery, The Affiliated Shaoyang Hospital of Traditional Chinese Medicine of Changsha Medical University, No. 631, Dongda Road, Shuangqing District, Shaoyang City, Hunan Province, 422000, China. axh19731030@163.com.
Yupei JiangDepartment of Clinical Laboratory, Shaoyang Hospital of TCM, Shaoyang City, Hunan Province, 422000, China.
Xiaoqing HuangDepartment of Tumor Surgery, Shaoyang Hospital of TCM, Shaoyang City, Hunan Province, 422000, China.
Haitao NingDepartment of Science and Education, Shaoyang Hospital of TCM, Shaoyang City, Hunan Province, 422000, China.
Shaojun WangDepartment of Clinical Laboratory, Shaoyang Hospital of TCM, Shaoyang City, Hunan Province, 422000, China.
Fang LiChangsha Medical University, Changsha City, Hunan Province, 410219, China.
Shulin WuChangsha Medical University, Changsha City, Hunan Province, 410219, China.
Jun MoDepartment of Hepatobiliary and Hernia Surgery, Anorectal, Second Affiliated Hospital of Shaoyang University, Shaoyang City, Hunan Province, 422000, China.

Funding

Neuro-oncology of Familial Neoplasia SyndromesZ01NS003053 · NINDS · NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE · PI LONSER, RUSSELL · 2008 to 2008
$1.0M
Intramural NIH HHS Z01 NS003053Key Project of Science and Technology Plan of Shaoyang City 2022NS3053
6 · The paper itself

Abstract

backgroundCancer cell stemness plays a decisive role in tumor metastasis and recurrence. TATA-box binding protein-associated factor 7 (TAF7), which is highly expressed in multiple cancers and critical for metastasis, has not been clearly linked to stemness characteristics in colorectal cancer (CRC). Therefore, this study aimed to investigate the role of TAF7 in CRC stemness.

methodsDifferential gene expression and associated signaling pathways were screened using single-cell transcriptomic data from CRC and adjacent normal tissues obtained from the GEO database and validated in CRC cells. A TAF7-knockdown CRC cell line was established to assess the impact of TAF7 on tumor cell stemness. The molecular mechanism of TAF7 in regulating CRC stemness was explored through bioinformatics analysis combined with chromatin immunoprecipitation and dual-luciferase reporter assays and validated by rescue experiments. Finally, a CRC xenograft mouse model was established to examine the effect of targeting TAF7 on tumor cell stemness and epithelial-mesenchymal transition (EMT).

resultsRe-analysis of single-cell transcriptomics data identified a TAF7

conclusionOur integrated single-cell transcriptomic and functional analyses reveal that TAF7 promotes CRC stemness and EMT progression via transcriptional activation of the stemness marker SOX2.

Indexed as

Colorectal NeoplasmsNeoplastic Stem CellsSOXB1 Transcription FactorsTATA-Binding Protein Associated FactorsTranscription Factor TFIIDAnimalsCell Line, TumorEpithelial-Mesenchymal TransitionGene Expression Regulation, NeoplasticHumansMiceSequence Analysis, RNASingle-Cell AnalysisSingle-Cell Gene Expression AnalysisSOX2 protein, humanSOXB1 Transcription FactorsTAF7 protein, humanTATA-Binding Protein Associated FactorsTranscription Factor TFIIDCancer cell stemnessColorectal cancerSingle-cell sequencingSOX2TAF7

Identifiers

PMID42897654
PMCPMC13679157

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.