Evidence map›Paper›PMID 42897585›Full record

ArticleBiotechnology for biofuels and bioproducts2026

A 2-pyrone-4,6-dicarboxylic acid biosensor for lignin valorization based on a newly identified transcriptional regulatory system.

Kenta Tanatani, Hyo Suzuki, Masaya Fujita, Takuma Araki, Yuzo Suzuki, Tsuyoshi Michinobu, Yuichiro Otsuka, Masaya Nakamura, Eiji Masai, Naofumi Kamimura

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In one paragraph

Article in Biotechnology for biofuels and bioproducts, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Kenta Tanatani *Department of Materials Science and Bioengineering, Nagaoka University of Technology, Nagaoka, Niigata, 940-2188, Japan.
Hyo Suzuki *Department of Materials Science and Bioengineering, Nagaoka University of Technology, Nagaoka, Niigata, 940-2188, Japan.
Masaya FujitaDepartment of Materials Science and Bioengineering, Nagaoka University of Technology, Nagaoka, Niigata, 940-2188, Japan.
Takuma ArakiDepartment of Forest Resource Chemistry, Forestry and Forest Products Research Institute, Tsukuba, Ibaraki, Japan.
Yuzo SuzukiDepartment of Forest Resource Chemistry, Forestry and Forest Products Research Institute, Tsukuba, Ibaraki, Japan.
Tsuyoshi MichinobuDepartment of Materials Science and Engineering, Institute of Science Tokyo, Meguro-Ku, Tokyo, Japan.
Yuichiro OtsukaDepartment of Forest Resource Chemistry, Forestry and Forest Products Research Institute, Tsukuba, Ibaraki, Japan.
Masaya NakamuraDepartment of Forest Resource Chemistry, Forestry and Forest Products Research Institute, Tsukuba, Ibaraki, Japan.
Eiji MasaiDepartment of Materials Science and Bioengineering, Nagaoka University of Technology, Nagaoka, Niigata, 940-2188, Japan.
Naofumi KamimuraDepartment of Materials Science and Bioengineering, Nagaoka University of Technology, Nagaoka, Niigata, 940-2188, Japan. zkami@vos.nagaokaut.ac.jp.ORCID https://orcid.org/0000-0002-8792-1675

Funding

JSPS J-PEAKS JPJS00420240017JSPS KAKENH 21K05872JSPS KAKENHI 24K01891JST COI-NEXT JPMJPF2104JST CREST JPMJCR23L4
6 · The paper itself

Abstract

backgroundLignin is the most abundant renewable aromatic resource on Earth, yet its efficient conversion into value-added chemicals through biological funneling remains a key challenge. Sphingobium lignivorans SYK-6 exhibits broad metabolic capacity for lignin-derived aromatic compounds, ranging from monomers to dimers, making it a suitable host for biological funneling. Production systems for the polymer building block 2-pyrone-4,6-dicarboxylic acid (PDC) from lignin-derived aromatic compounds have been constructed using SYK-6. In this study, we developed a PDC-responsive biosensor to facilitate high-throughput screening of improved PDC-producing strains.

resultsPDC-assimilating bacteria were isolated from environmental samples, and their genomes were analyzed to obtain candidate PDC-responsive transcriptional regulatory systems. Among these, the LysR-type transcriptional regulator PdcR and its target promoter P

conclusionsΔligI(pCup1) enables real-time, specific detection of PDC produced from lignin-derived aromatic compounds, including those in black liquor extract derived from lignocellulosic biomass, demonstrating applicability to screening for lignin valorization. Integration of this biosensor with diverse strain engineering approaches, including random mutagenesis, adaptive laboratory evolution, and metagenomics-based enzyme discovery, would provide a platform for efficient selection of SYK-6 strains with enhanced PDC production capability.

Indexed as

2-Pyrone-4,6-dicarboxylic acid (PDC)Biological funnelingBiosensorHigh-throughput screeningLignin valorizationSphingobium lignivorans SYK-6

Identifiers

PMID42897585
PMCPMC13681972

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.