Evidence map›Paper›PMID 42897262›Full record

ArticleCellular and molecular life sciences : CMLS2026

Deciphering the heterogeneity of chicken gonadal primordial germ cells via single-cell RNA sequencing.

Xiaomin Wang, Shenghu Zhang, Kaipeng Wang, Qianru Li, Yihang Yang, Xingnan Huang, Zhuangfei Wang, Mengmeng Guo, Bo Chen, Zheting Zhang and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cellular and molecular life sciences : CMLS, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

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2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Xiaomin WangLaboratory of Cell Fate Control, School of Life Sciences, Westlake University, Hangzhou, China. wangxiaomin@westlake.edu.cn.
Shenghu ZhangLaboratory of Cell Fate Control, School of Life Sciences, Westlake University, Hangzhou, China.
Kaipeng WangLaboratory of Cell Fate Control, School of Life Sciences, Westlake University, Hangzhou, China.
Qianru LiLaboratory of Cell Fate Control, School of Life Sciences, Westlake University, Hangzhou, China.
Yihang YangLaboratory of Cell Fate Control, School of Life Sciences, Westlake University, Hangzhou, China.
Xingnan HuangLaboratory of Cell Fate Control, School of Life Sciences, Westlake University, Hangzhou, China.
Zhuangfei WangLaboratory of Cell Fate Control, School of Life Sciences, Westlake University, Hangzhou, China.
Mengmeng GuoLaboratory of Cell Fate Control, School of Life Sciences, Westlake University, Hangzhou, China.
Bo ChenLaboratory of Cell Fate Control, School of Life Sciences, Westlake University, Hangzhou, China.
Zheting ZhangLaboratory of Cell Fate Control, School of Life Sciences, Westlake University, Hangzhou, China.
Bo WangZhejiang University of Science and Technology School of Information and Electronic Engineering, Hangzhou, China. wangbo@westlake.edu.cn.
Duanqing PeiLaboratory of Cell Fate Control, School of Life Sciences, Westlake University, Hangzhou, China. peiduanqing@westlake.edu.cn.
Chengchen ZhaoLaboratory of Cell Fate Control, School of Life Sciences, Westlake University, Hangzhou, China. zhaochengchen@westlake.edu.cn.ORCID http://orcid.org/0000-0002-8454-6347

Funding

the Pioneer and Leading Goose R&D Program of Zhejiang 2025C01115the Yangtze River Delta Sci-Tech Innovation Community Joint Research Project 2022CSJGG1000the Zhejiang Provincial Natural Science Foundation of China LZ25C060003
6 · The paper itself

Abstract

Chicken primordial germ cells (PGCs) are valuable for breeding, germplasm preservation, and genetic modifications. Here, using single-cell transcriptome analysis, we found that three transcriptionally distinct PGC subtypes were exist in chicken embryonic gonads PGCs. We further demonstrated that in vitro-cultured PGCs could integrate into recipient gonads via blood vessel injection. Additionally, the gene-modified PGCs could be re-established and integrated into recipient gonads after the PGCs were cultured for long-term use by a second blood-vessel injection. Together, these results provide evidence for substantial heterogeneity among chicken gonadal PGCs, which were previously considered a homogeneous cell population. This diversity could be leveraged to establish and expand PGCs for long-term in vitro culture from differentiated gonads.

Indexed as

Germ CellsGonadsSingle-Cell AnalysisAnimalsCell DifferentiationChick EmbryoChickensGene Expression ProfilingGene Expression Regulation, DevelopmentalSequence Analysis, RNASingle-Cell Gene Expression AnalysisTranscriptomeAvian germlineChimeric chickensGonadal developmentRe-established PGC linesTranscriptomic heterogeneity

Identifiers

PMID42897262
PMCPMC13680580

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.