ArticleZhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery2026
[Biological characteristics of urine-derived stem cells during
Article in Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Objective: To explore the biological characteristics of urine-derived stem cells (USCs) during Methods: USCs were isolated from fresh midstream urine samples from healthy volunteers by using the centrifugation combined with adherent cultivation method, followed by continuous passaging at a 1∶3 ratio. The morphological changes of cells were observed under inverted microscopy and analyzed using Image J software. After osteogenic and chondrogenic induction and identification of cells at passage 3 (P3), the biological characteristics of cells at different passages (P3, P5, and P7) were compared. Cell proliferation, cell cycle, senescence, and apoptosis were assessed via cell counting kit 8 assays, flow cytometry, β-galactosidase staining, and Annexin Ⅴ-fluorescein isothiocyanate/propidium iodide staining. RT-PCR was used to detect the expression of stemness genes (Nanog, Oct4, c-Myc, and Sox2). Tube formation assays were conducted to evaluate the Results: After osteogenic and chondrogenic induction, USCs were positive for alkaline phosphatase, Alizarin red, and Alcian blue staining, respectively. During expansion, the morphology of USCs gradually shifted from rice-grain-like to spindle-shaped, with increasing trends in long diameter, short diameter, area, and aspect ratio ( Conclusion: During
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