ArticleMethodsX2026
Simultaneous determination of serotonin and its precursor tryptophan in small volumes of human serum using HPLC-ECD.
Article in MethodsX, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Authors and funding
5 authors.
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Abstract
Serotonin (5-HT) is not only a key neurotransmitter in the brain but also plays an important role in peripheral tissues e.g. in the gastrointestinal (GI) tract. The multitude of processes in which 5-HT is involved explains why recent studies have linked altered 5-HT levels to Long Covid (LC) and fatigue. LC is a post-viral syndrome which can occur after a SARS-CoV-2 infection with various symptoms, amongst others including fatigue. We present an optimized bioanalytical HPLC method with electrochemical detection (ECD) for the simultaneous detection of 5-HT and its precursor tryptophan (W) in small volumes of human serum. We validated the method with a calibration curve better representing concentrations expected in human serum; with the possibility to concurrently measure W and low 5-HT concentrations. Sample comparability and standardization were improved through an adapted sample preparation, reducing transfer errors and increasing recovery of the internal standard (IS). The simple preparation involved only two steps: protein precipitation and sample dilution. After a brief series of validation measurements of the optimized method, it was applied to human serum from an observational study cohort.•Easy-to-use method for detecting 5-HT and W in human serum without the need for expensive buffer ingredients or pre-filter units.•Enables measurement of triplicates with less than 100 µL serum.
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