Evidence map›Paper›PMID 42891715›Full record

ArticleMarine biotechnology (New York, N.Y.)2026

Lipid Extract from Ulva ohnoi Algae: Chemical Characterization, Antibacterial Potential, and Cytotoxicity in the Search for New Agents Against MDR Infections.

Marisa Castro Jara, Diego Serrasol do Amaral, Leonardo Werner, Eduardo Bastos, Luana de Azevedo Aimi, Nathalia Stark Pedra, Vithor Parada Garcia, Matheus Pereira de Albuquerque, Clarissa Piccinin Frizzo, Milena de Nardin and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Marine biotechnology (New York, N.Y.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Marisa Castro JaraDepartment of Microbiology and Parasitology, Institute of Biology, Federal University of Pelotas, Pelotas, RS, Brazil. marisa.jara@ufpel.edu.br.ORCID http://orcid.org/0000-0002-9437-3558
Diego Serrasol do AmaralDepartment of Biochemistry and Bioprospecting, Federal University of Pelotas, Pelotas, RS, Brazil.ORCID http://orcid.org/0000-0002-5436-2870
Leonardo WernerDepartment of Biochemistry and Bioprospecting, Federal University of Pelotas, Pelotas, RS, Brazil.ORCID http://orcid.org/0009-0000-2027-6037
Eduardo BastosDepartment of Botany, Federal University of Santa Catarina, Florianópolis, SC, Brazil.ORCID http://orcid.org/0000-0001-6710-7281
Luana de Azevedo AimiDepartment of Botany, Federal University of Santa Catarina, Florianópolis, SC, Brazil.ORCID http://orcid.org/0009-0004-8682-7910
Nathalia Stark PedraLaboratory of Neurochemistry, Inflammation, and Cancer, Federal University of Pelotas, Pelotas, RS, Brazil.ORCID http://orcid.org/0000-0001-8662-7661
Vithor Parada GarciaLaboratory of Research in Biochemistry and Molecular Biology of Microorganisms, Federal University of Pelotas, Pelotas, RS, Brazil.ORCID http://orcid.org/0009-0005-1834-6370
Matheus Pereira de AlbuquerqueLaboratory of Research in Biochemistry and Molecular Biology of Microorganisms, Federal University of Pelotas, Pelotas, RS, Brazil.ORCID http://orcid.org/0009-0007-2175-2374
Clarissa Piccinin FrizzoLaboratory of Ionic Liquids and Nanomaterials, Department of Chemistry, Federal University of Santa Maria, Santa Maria, RS, Brazil.ORCID http://orcid.org/0000-0002-1175-7880
Milena de NardinLaboratory of Ionic Liquids and Nanomaterials, Department of Chemistry, Federal University of Santa Maria, Santa Maria, RS, Brazil.ORCID http://orcid.org/0009-0004-9888-5499
Wellington da Rocha da SilvaDepartment of Preventive Veterinary Medicine, Faculty of Veterinary Medicine, Federal University of Pelotas, Pelotas, RS, Brazil.ORCID http://orcid.org/0000-0001-7460-1376
Silvia de Oliveira HübnerDepartment of Preventive Veterinary Medicine, Faculty of Veterinary Medicine, Federal University of Pelotas, Pelotas, RS, Brazil.ORCID http://orcid.org/0000-0003-0482-9599
Rodrigo de Almeida VaucherLaboratory of Research in Biochemistry and Molecular Biology of Microorganisms, Federal University of Pelotas, Pelotas, RS, Brazil.ORCID http://orcid.org/0000-0003-3496-7335
Cláudio Martins Pereira de PereiraDepartment of Biochemistry and Bioprospecting, Federal University of Pelotas, Pelotas, RS, Brazil.ORCID http://orcid.org/0000-0002-2818-4281
Patrícia da Silva NascenteDepartment of Microbiology and Parasitology, Institute of Biology, Federal University of Pelotas, Pelotas, RS, Brazil.ORCID http://orcid.org/0000-0002-3137-4107

Funding

Conselho Nacional de Desenvolvimento Científico e Tecnológico , Brasil 88887185292/2025-00Coordenação de Aperfeiçoamento de Pessoal de Nível Superior , Brasil 001Delfim Mendes Silveira Foundation 3058/2025
6 · The paper itself

Abstract

Antimicrobial resistance is a critical global concern. This study explored the lipid extract (LE) of Ulva ohnoi regarding its chemical composition, antibacterial activity, and cytotoxicity against multidrug-resistant (MDR) microorganisms. The LE was characterized by GC-MS, thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC). Antibacterial efficacy was determined by Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) against MDR isolates: Acinetobacter baumannii, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus, and coagulase-negative Staphylococci (CoNS). Cytotoxicity was evaluated in murine fibroblast (L929) and bovine renal fibroblast (MDBK) cells using MTT, Neutral Red (NR), Lactate Dehydrogenase (LDH), and hemolytic activity (HA) assays. Lipid characterization revealed palmitic acid (63.62%) and oleic acid (21.01%) as major components. TGA indicated 88% mass loss, and DSC showed no amorphous/crystalline domains. LE showed favorable MIC values against S. aureus and CoNS (1.33 mg/mL) and against A. baumannii and E. coli (2 mg/mL), indicating moderate antibacterial activity typical of an unfractionated crude extract. MBC results indicated bacteriostatic activity (1-8 mg/mL). The extract was cytotoxic to L929 and MDBK cells at concentrations close to its MIC (IC₅₀ 0.25-1.95 mg/mL) but well tolerated by blood cells (HA IC

Indexed as

Anti-Bacterial AgentsLipidsPlant ExtractsUlvaAnimalsCattleCell LineDrug Resistance, Multiple, BacterialEscherichia coliGas Chromatography-Mass SpectrometryMiceMicrobial Sensitivity TestsStaphylococcus aureusAnti-Bacterial AgentsLipidsPlant ExtractsAntibioticsChlorophytaGreen macroalgaeGreen tideSea lettuceSeaweed

Identifiers

PMID42891715
PMCPMC13676642

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.