Evidence map›Paper›PMID 42891309›Full record

ArticleiMetaOmics2026

Metagenomic mining reveals novel Cas12 subtypes and their evolutionary diversification.

Meixia Yu, Zilong Zhao, Peiyang Li, Yunpeng Teng, Feng Xu, Zhimeng Li, Zhijia Yang, Hainan Zhao, Huanbin Zhou, Lanqin Xia and 2 more

Abstract readLetter
PubMed Full text at PMC Publisher
In one paragraph

Article in iMetaOmics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Meixia YuState Key Laboratory of Maize Bio-breeding, National Maize Improvement Center, Department of Plant Genetics and Breeding China Agricultural University Beijing China.
Zilong ZhaoState Key Laboratory of Maize Bio-breeding, National Maize Improvement Center, Department of Plant Genetics and Breeding China Agricultural University Beijing China.
Peiyang LiState Key Laboratory of Maize Bio-breeding, National Maize Improvement Center, Department of Plant Genetics and Breeding China Agricultural University Beijing China.
Yunpeng TengState Key Laboratory of Maize Bio-breeding, National Maize Improvement Center, Department of Plant Genetics and Breeding China Agricultural University Beijing China.
Feng XuState Key Laboratory of Maize Bio-breeding, National Maize Improvement Center, Department of Plant Genetics and Breeding China Agricultural University Beijing China.
Zhimeng LiState Key Laboratory of Maize Bio-breeding, National Maize Improvement Center, Department of Plant Genetics and Breeding China Agricultural University Beijing China.
Zhijia YangFrontiers Science Center for Molecular Design Breeding (Ministry of Education) China Agricultural University Beijing China.
Hainan ZhaoFrontiers Science Center for Molecular Design Breeding (Ministry of Education) China Agricultural University Beijing China.
Huanbin ZhouState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing China.
Lanqin XiaMinistry of Agriculture and Rural Affairs Key Laboratory of Gene Editing Technologies (Hainan), National Nanfan Research Institute Chinese Academy of Agricultural Sciences Sanya China.
Jinsheng LaiState Key Laboratory of Maize Bio-breeding, National Maize Improvement Center, Department of Plant Genetics and Breeding China Agricultural University Beijing China.
Beibei XinState Key Laboratory of Maize Bio-breeding, National Maize Improvement Center, Department of Plant Genetics and Breeding China Agricultural University Beijing China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We identified 4112 Cas12 Proteins and 6 new Cas12 subtypes, revealed their significant diversity in N-terminal regions, repeat sequences, and sequences motifs. We developed an AI-driven algorithm, Cas12fam, which allows precise annotation of 18 distinct domains in Cas12 proteins. Through detailed structural analysis, we identified two novel glycine residues in the RuvC-like nuclease domains (RuvC-I, RuvC-II), which were experimentally validated to play a critical role in the DNA-cleavage activity of Cas12. Our analyses revealed that Cas12 proteins are enriched in specific environments, with significant variation in their abundance across ecological niches.

Identifiers

PMID42891309
PMCPMC13674389

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.