ArticleFrontiers in microbiology2026
Alfalfa fields with different stand ages alters soil inorganic nitrogen dynamics through shifts in nitrogen-cycling functional genes across growth stages.
Article in Frontiers in microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Introduction: Alfalfa cultivation is associated with soil nitrogen availability and microbial nitrogen-cycling functional potential. However, how soil inorganic nitrogen and nitrogen-cycling functional genes vary with alfalfa stand age across growth stages remains unclear. Methods: Using a chronosequence approach, we investigated soil inorganic nitrogen and nitrogen-cycling functional genes in a maize-field control (CK) and 2-, 3-, 4-, and 7-year-old alfalfa fields (T1-T4) during the regreening, flowering, and maturity-harvest stages. Soil Results: Soil Conclusion: Overall, the 4-year alfalfa field exhibited the highest observed soil inorganic nitrogen concentrations, while this pattern was less evident in the 7-year field. These findings indicate that soil nitrogen status and nitrogen-cycling functional genes vary jointly with alfalfa stand age and growth stage. Nitrogen-cycling functional genes may serve as potential indicators of soil nitrogen status, although further experimental studies are needed to verify these relationships.
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