ArticleFrontiers in immunology2026
Identification of heterogeneous subtypes of human dendritic cells from the lymph nodes of patients with gastric cancer.
Article in Frontiers in immunology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Objective: The subtypes of heterogeneous conventional dendritic cells (cDCs) and plasmacytoid dendritic cells (pDCs) in lymph nodes (LN) cannot be distinguished because they share similar surface markers. This study aimed to identify cDCs, pDCs, and other cell types in DC-enriched LNs from patients with gastric cancer (GC) by analyzing surface markers and gene expression. Methods: Expression of surface markers, including CD141, CD1c, and CD123, in DC-enriched cells was analyzed by conventional flow cytometry and AbSeq using antibody-derived tags. Transcriptome and epitope analyses were used to characterize DC subtypes. The frequencies of heterogeneous DC subtypes were compared across clinical stages and molecular subtypes in patients with GC. Results: DC-enriched cells showed CD1c Conclusion: Cluster analysis of DC-enriched cells in LNs from our integrated dataset revealed subtypes of the cDC1, cDC2, and pDC lineages. We identified distinctive DC subtypes in the transitional state bridging differentiation between cDC1 and cDC2. CD137-DC may be an independent DC subset with mature and migratory features.
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