Evidence map›Paper›PMID 42889615›Full record

ArticleJournal of extracellular biology2026

Evaluating Plasma-Handling Effects on Extracellular Vesicle RNA Yield and Cargo Profiles in Fish: A Methodological Insight.

Antonio Espuela-Ortiz, Estrella Antón-González, Louis Bonnefous, M Virginia Martín, Salvador Jeréz, Deiene Rodríguez-Barreto

Abstract read
In one paragraph

Article in Journal of extracellular biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Antonio Espuela-OrtizDepartment of Animal Biology, Soil Science and Geology Universidad de La Laguna San Cristóbal de La Laguna Tenerife Spain.ORCID https://orcid.org/0000-0003-0572-9549
Estrella Antón-GonzálezDepartment of Animal Biology, Soil Science and Geology Universidad de La Laguna San Cristóbal de La Laguna Tenerife Spain.
Louis BonnefousDepartment of Animal Biology, Soil Science and Geology Universidad de La Laguna San Cristóbal de La Laguna Tenerife Spain.
M Virginia MartínCanary Islands Oceanographic Center (COC) IEO-CSIC Santa Cruz de Tenerife Spain.
Salvador JerézCanary Islands Oceanographic Center (COC) IEO-CSIC Santa Cruz de Tenerife Spain.
Deiene Rodríguez-BarretoDepartment of Animal Biology, Soil Science and Geology Universidad de La Laguna San Cristóbal de La Laguna Tenerife Spain.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Extracellular vesicle (EV)-derived RNA (EV-RNA) is emerging as a valuable biomarker source in aquatic species. However, the composition and stability of long RNA species, particularly messenger RNA (mRNAs) and long non-coding RNAs (lncRNAs), remain largely unexplored in teleosts. Pre-analytical factors such as input plasma volume and storage conditions may influence RNA recovery, especially in low-input samples like fish plasma. Here, we evaluated the effects of input sample volume and freezing storage on EV-RNA yield and integrity in gilthead sea bream (

Indexed as

exoRNeasyextracellular vesiclefreeze‐thawgilthead sea breamkit formatMinION Mk1COxford Nanopore TechnologyplasmaSparus auratateleost fish

Identifiers

PMID42889615
PMCPMC13670421

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.