Evidence map›Paper›PMID 42888406›Full record

ArticleBMC microbiology2026

Prophylactic administration of probiotic strains Weizmannia coagulans and Saccharomyces boulardii attenuates early dysplastic changes in an AOM/DSS-induced mouse model.

Marwa Matboli, Sanaa Eissa, Reham Hussein Mohamed, Nada Kotb, Ghada Galal Hamam, Mai Tarek, Nagwa M Abo El Magd, Abdullah M Abdo, Aisha Mohamed Hussein, Naydeen Mostafa and 1 more

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Article in BMC microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Marwa MatboliMedical Biochemistry and Molecular Biology Department, Faculty of Medicine, Ain Shams University, P.O. box 11381, Abbassia, Cairo, Egypt. DrMarwa_Matboly@med.asu.edu.eg.ORCID http://orcid.org/0000-0002-6852-3954
Sanaa EissaMedical Biochemistry and Molecular Biology Department, Faculty of Medicine, Ain Shams University, P.O. box 11381, Abbassia, Cairo, Egypt. drsanaa_mohamed@med.asu.edu.eg.ORCID http://orcid.org/0000-0001-8591-3244
Reham Hussein MohamedClinical Pharmacology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
Nada KotbClinical Pharmacology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
Ghada Galal HamamDepartment of Medical Histology, Faculty of Medicine, Ain Shams University, Cairo, Egypt.ORCID http://orcid.org/0000-0001-6562-2132
Mai TarekDepartment of Medical Histology, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
Nagwa M Abo El MagdDepartment of Medical Microbiology and Immunology, Faculty of Medicine, Ain Shams University, Cairo, Egypt.ORCID http://orcid.org/0000-0003-0705-8240
Abdullah M AbdoBotany and Microbiology Department, Faculty of Science, Al-Azhar University, P.O. Box 11884, Nasr City, Cairo, Egypt.ORCID http://orcid.org/0000-0003-0087-0674
Aisha Mohamed HusseinBiochemistry and Molecular Biology Department, Oncology Diagnostic Research Unit (ODU), Faculty of Medicine, Ain Shams University, Cairo, Egypt.
Naydeen MostafaFaculty of Medicine, Ain Shams University, Cairo, Egypt.
Marwa Mostafa KamelMedical Biochemistry and Molecular Biology Department, Faculty of Medicine, Ain Shams University, P.O. box 11381, Abbassia, Cairo, Egypt.ORCID http://orcid.org/0000-0001-5622-0887

Funding

STDF-Science and Technology Development Fund for accredited centers of scientific excellence, Cairo, Egypt (Grant no. 4118 51). STDF-Science and Technology Development Fund for accredited centers of scientific excellence, Cairo, Egypt (Grant no. 4118 51).
6 · The paper itself

Abstract

backgroundColorectal dysplasia represents an early stage of colorectal carcinogenesis and is closely associated with chronic inflammation and altered microRNA (miRNA) expression. Probiotic strains such as Weizmannia coagulans (formerly Bacillus coagulans) and Saccharomyces boulardii possess anti-inflammatory properties and may influence host miRNA expression, suggesting their potential as prophylactic agents against inflammation-associated colorectal dysplasia.

methodsMice received prophylactic oral administration of Weizmannia coagulans or Saccharomyces boulardii beginning one week before azoxymethane/dextran sulfate sodium (AOM/DSS) induction and continuing throughout the experimental period. Histopathological examination, TNF-α immunohistochemistry, serum pro-inflammatory cytokine measurements, and miRNA expression profiling using reverse transcription quantitative polymerase chain reaction (RT-qPCR) were performed to evaluate the prophylactic effects of both probiotic strains.

resultsProphylactic administration of both probiotic strains significantly attenuated early dysplastic alterations and inflammatory cell infiltration compared with the AOM/DSS group (p < 0.05). These histopathological improvements were accompanied by significantly lower serum levels of the pro-inflammatory cytokines IL-6 and IL-1β, together with reduced TNF-α immunoreactivity (p < 0.05). In addition, probiotic pretreatment attenuated the altered miRNA expression profile induced by AOM/DSS. Compared with the model group, expression of miR-122-5p, miR-29b-5p, and miR-15b-5p was significantly reduced, whereas miR-98-5p, miR-202-5p, miR-21-5p, and let-7i-5p showed increased expression and were associated with miRNA expression patterns that more closely resembled those of the control group (p < 0.01).

conclusionProphylactic administration of Weizmannia coagulans and Saccharomyces boulardii reduced inflammation-associated early dysplastic changes while preserving normal miRNA expression patterns in an AOM/DSS-induced murine model. These findings support the potential of probiotic prophylaxis as an early preventive strategy against colorectal dysplasia and provide a foundation for future studies investigating its clinical applicability.

Indexed as

Colorectal NeoplasmsProbioticsSaccharomyces boulardiiAnimalsAzoxymethaneCytokinesDextran SulfateDisease Models, AnimalMaleMiceMicroRNAsTumor Necrosis Factor-alphaAzoxymethaneCytokinesDextran SulfateMicroRNAsTumor Necrosis Factor-alphaAOM/DSS mouse modelColorectal dysplasiaInflammationmiRNAsProbioticsqRT-PCRSaccharomyces boulardiiWeizmannia coagulans

Identifiers

PMID42888406

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.