Evidence map›Paper›PMID 42888239›Full record

ReviewNature methods2026

Recent developments of fluorescent proteins and biosensors driven by cross-disciplinary insights.

Anneliese M M Gest, Sohum Mehta, Jin Zhang

Abstract readReview
PubMed Publisher
In one paragraph

Review in Nature methods, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Anneliese M M GestDepartment of Pharmacology, University of California, San Diego, La Jolla, CA, USA.
Sohum MehtaDepartment of Pharmacology, University of California, San Diego, La Jolla, CA, USA.
Jin ZhangDepartment of Pharmacology, University of California, San Diego, La Jolla, CA, USA. jzhang32@ucsd.edu.ORCID http://orcid.org/0000-0001-7145-7823

Funding

UCSD/SDSU IRACDAK12GM068524 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI TREJO, JOANN · 2003 to 2025
$24.4M
Live-cell Activity Architecture in CancerR35CA197622 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Jin Zhang · 2015 to 2026
$11.2M
Mechanisms of Compartmentalized cAMP SignalingR01DK073368 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Jin Zhang · 2006 to 2026
$7.6M
Ultrasensitive kinase biosensors for multiplex imaging of coordinated spatiotemporal signaling in cancer-immune interactionsR01CA262815 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Yingxiao Wang, Jin Zhang · 2022 to 2026
$3.3M
AMPK Regulation of ACE2 in Endothelial Health and DiseaseR01HL162302 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI SHYY, JOHN YJ, ZHANG, JIN · 2022 to 2025
$2.7M
Development of kinase biosensors for multiplex neuronal imaging of signaling pathways in behaving miceRF1MH126707 · NIMH · JOHNS HOPKINS UNIVERSITY · PI HUGANIR, RICHARD L, ZHANG, JIN · 2022 to 2022
$2.4M
Signal Transduction by PI3K/mTORR01DE030497 · NIDCR · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI GUTKIND, JORGE SILVIO, ZHANG, JIN · 2021 to 2025
$2.4M
U.S. Department of Health & Human Services | NIH | National Cancer Institute (NCI) R01 CA262815U.S. Department of Health & Human Services | NIH | National Cancer Institute (NCI) R35 CA197622U.S. Department of Health & Human Services | NIH | National Heart, Lung, and Blood Institute (NHLBI) R01 HL162302U.S. Department of Health & Human Services | NIH | National Heart, Lung, and Blood Institute (NHLBI) T32 5T32HL086344U.S. Department of Health & Human Services | NIH | National Institute of Dental and Craniofacial Research (NIDCR) R01 DE030497U.S. Department of Health & Human Services | NIH | National Institute of Diabetes and Digestive and Kidney Diseases (National Institute of Diabetes & Digestive & Kidney Diseases) R01 DK073368U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (NIGMS) K12 GM068524U.S. Department of Health & Human Services | NIH | National Institute of Mental Health (NIMH) RF1MH126707
6 · The paper itself

Abstract

Since the discovery and application of green fluorescent protein for use as an optical tag, fluorescent proteins (FPs) have changed the way we observe biological signals and processes. FP and fluorescent biosensor design has continued to advance, facilitated by new technologies and developments in chemistry, physics, engineering and screening technologies. We summarize some of the recent developments in FP engineering, focusing on insights that have led to improved photostability or longer wavelengths, among other improvements, highlighting mScarlet as a case study. We also discuss the engineering of new fluorescent biosensors, focusing on the ways in which screening, quantitative imaging, spatial resolution and multiplexing have been improved in recent years. Finally, we conclude with a perspective on what the future holds for the next generation of FPs and biosensors.

Identifiers

PMID42888239

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.