Evidence map›Paper›PMID 42884663›Full record

ArticleNutrients2026

Maternal Obesity Induces DNA Methylation Changes in the Kidneys and Blood of Rodent Offspring at Adulthood.

Benjamin P Larkin, Long T Nguyen, Naveen K Parthiban, Hui Chen, Alen Faiz, Kushalee Jayawickreme, Sarah Glastras, Carol A Pollock, Sonia Saad

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Article in Nutrients, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Benjamin P LarkinRenal Research Laboratory, Level 9, Kolling Institute of Medical Research, Royal North Shore Hospital, University of Sydney, St. Leonards, Sydney, NSW 2065, Australia.
Long T NguyenRenal Research Laboratory, Level 9, Kolling Institute of Medical Research, Royal North Shore Hospital, University of Sydney, St. Leonards, Sydney, NSW 2065, Australia.ORCID 0000-0002-0630-4959
Naveen K ParthibanRenal Research Laboratory, Level 9, Kolling Institute of Medical Research, Royal North Shore Hospital, University of Sydney, St. Leonards, Sydney, NSW 2065, Australia.
Hui ChenSchool of Life Sciences, Faculty of Science, University of Technology Sydney, Ultimo, Sydney, NSW 2007, Australia.ORCID 0000-0001-6883-3752
Alen FaizSchool of Life Sciences, Faculty of Science, University of Technology Sydney, Ultimo, Sydney, NSW 2007, Australia.
Kushalee JayawickremeRenal Research Laboratory, Level 9, Kolling Institute of Medical Research, Royal North Shore Hospital, University of Sydney, St. Leonards, Sydney, NSW 2065, Australia.
Sarah GlastrasRenal Research Laboratory, Level 9, Kolling Institute of Medical Research, Royal North Shore Hospital, University of Sydney, St. Leonards, Sydney, NSW 2065, Australia.ORCID 0000-0002-9317-1348
Carol A PollockRenal Research Laboratory, Level 9, Kolling Institute of Medical Research, Royal North Shore Hospital, University of Sydney, St. Leonards, Sydney, NSW 2065, Australia.
Sonia SaadRenal Research Laboratory, Level 9, Kolling Institute of Medical Research, Royal North Shore Hospital, University of Sydney, St. Leonards, Sydney, NSW 2065, Australia.ORCID 0000-0001-8067-8046

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

aimTo identify differentially methylated genes in offspring of obese versus lean mothers using a mouse model of maternal obesity.

backgroundAdverse conditions during fetal development predispose offspring to adult diseases, supporting the theory of fetal programming. DNA methylation is suggested as a mechanism by which maternal obesity induces fetal programming. As maternal obesity predisposes offspring to chronic kidney disease (CKD), we aimed to investigate the role of DNA methylation in maternal obesity related to CKD in the offspring.

methodsMaternal obesity was modelled by feeding female C57BL/6 mice a high-fat diet before breeding, and during gestation and lactation. Control dams received chow. Male offspring were weaned to chow on postnatal day 20 and were sacrificed at 32 weeks of age (adulthood). Another cohort was sacrificed at birth. Reduced representation bisulfite sequencing was used to identify differentially methylated genes (DMGs) in the offspring kidney and blood at adulthood and at birth. Real-time PCR was used to determine gene expression.

resultsAdult offspring of obese dams showed significant DMGs in both kidney and blood at adulthood in association with increased levels of kidney injury. Among these, 10 genes were differentially methylated in the kidney, out of which, four genes were also differentially methylated in the blood. An additional 10 genes were differentially methylated in the blood only. Renal mRNA expression of four genes (

conclusionsMaternal obesity induced differential gene methylation in the kidneys and blood of adult offspring. Several of these genes have shown high relevance to kidney fibrosis, diabetes or obesity, supporting the role of epigenetic regulation in fetal programming to CKD by maternal obesity.

Indexed as

DNA MethylationKidneyMaternal Nutritional Physiological PhenomenaObesityPregnancy in ObesityPrenatal Exposure Delayed EffectsAnimalsDevelopmental Origins of Health and DiseaseDiet, High-FatEpigenesis, GeneticFemaleMaleMiceMice, Inbred C57BLPregnancyRenal Insufficiency, Chronicchronic kidney diseaseDNA methylationepigeneticsfetal programmingmaternal obesity

Identifiers

PMID42884663
PMCPMC13667289

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