ArticleNutrients2026
Folic Acid Oversupplementation Alters Global DNA Methylation and Circadian Clock Gene Expression in FaDu Head and Neck Squamous Cell Carcinoma Cells: An Exploratory Study.
Article in Nutrients, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
BACKGROUND/
objectivesFolic acid (FA), the synthetic form of folate (vitamin B9), is a vitamin involved in one-carbon metabolism, DNA synthesis, and DNA methylation. Because folate metabolism influences methyl donor availability, FA availability may affect circadian clock gene regulation through methylation-related or other metabolic mechanisms in head and neck squamous cell carcinoma (HNSCC). This exploratory study aims to determine whether FA oversupplementation influences cell viability and alters global DNA methylation, as well as the expression and methylation status of circadian clock genes in HNSCC cells.
methodsFaDu HNSCC cells were treated with 20 or 80 μM FA and compared with a no-added-FA (NF) reference condition. Cell viability was assessed using a CCK-8 assay after 48 and 72 h. For methylation and gene expression analyses, cells were synchronized with forskolin and treated with FA (20 or 80 μM) for 7 days. Global DNA methylation was measured using a 5-methylcytosine colorimetric assay. Following the NF and 80 μM FA treatment, cytosine-phosphate-guanine (CpG) island methylation in the
resultsFA treatment significantly reduced cell viability at 48 h (20 μM,
conclusionsThese exploratory findings suggest that the observed transcriptional changes are unlikely to be explained by methylation changes within the analyzed promoter regions of
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.