Evidence map›Paper›PMID 42884101›Full record

ArticleInternational journal of molecular sciences2026

Heterologous Expression and Immunoreactivity of Wheat Amylase-Trypsin Inhibitors.

Lisa Call, Thomas Svoboda, Andrea Krisai, Tim Hacker, Kathrin Lauter, Stefano D'Amico, Birgit Linhart, Agnès Amouric, Mickaël Lafond, Thierry Giardina and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Lisa CallDepartment of Agricultural Sciences, BOKU University, 3430 Tulln an der Donau, Austria.
Thomas SvobodaDepartment of Agricultural Sciences, BOKU University, 3430 Tulln an der Donau, Austria.ORCID 0000-0003-0502-301X
Andrea KrisaiDepartment of Agricultural Sciences, BOKU University, 3430 Tulln an der Donau, Austria.ORCID 0000-0001-6316-0536
Tim HackerDepartment of Agricultural Sciences, BOKU University, 3430 Tulln an der Donau, Austria.ORCID 0009-0007-9116-034X
Kathrin LauterDepartment of Agricultural Sciences, BOKU University, 3430 Tulln an der Donau, Austria.ORCID 0000-0002-6258-2777
Stefano D'AmicoAGES-Austrian Agency for Health and Food Safety, 1220 Vienna, Austria.
Birgit LinhartInstitute of Pathophysiology and Allergy Research, Medical University of Vienna, 1090 Vienna, Austria.
Agnès AmouriciSm2, CNRS, Aix-Marseille Université, Centrale Marseille, 13397 Marseille, France.ORCID 0000-0002-0315-7455
Mickaël LafondiSm2, CNRS, Aix-Marseille Université, Centrale Marseille, 13397 Marseille, France.ORCID 0000-0003-2646-0286
Thierry GiardinaiSm2, CNRS, Aix-Marseille Université, Centrale Marseille, 13397 Marseille, France.
Motohiro EbisawaClinical Research Center for Allergy and Rheumatology, NHO Sagamihara National Hospital, Sagamihara 252-0392, Kanagawa, Japan.
Rudolf ValentaInstitute of Pathophysiology and Allergy Research, Medical University of Vienna, 1090 Vienna, Austria.
Heinrich GrausgruberDepartment of Agricultural Sciences, BOKU University, 3430 Tulln an der Donau, Austria.ORCID 0000-0003-4790-0922

Funding

Government of Lower Austria K3-T-74/001-2019Institut francaise d'Autriche
6 · The paper itself

Abstract

Wheat amylase-trypsin inhibitors (ATIs) are known for their allergenic properties, but their precise role in celiac disease and other wheat-related disorders remains unclear. Pure ATI preparations provide a basis for detailed biochemical and immunologic characterization; however, the complexity of isolating ATIs in the absence of other immunoreactive wheat proteins is an ongoing challenge. To address this limitation, bacterial and yeast heterologous expression systems were evaluated for the production of pure ATI preparations. While the synthesis of recombinant ATIs 0.28 and CM3 in

Indexed as

AmylasesPlant ProteinsTriticumTrypsin InhibitorsEscherichia coliGene ExpressionHumansPichiaRecombinant ProteinsSaccharomycetalesAmylasesPlant ProteinsRecombinant ProteinsTrypsin Inhibitorsamylase–trypsin inhibitorsheterologous expression systemPichia pastorisrecombinant protein expressionTriticum sp.wheat-related disorders

Identifiers

PMID42884101
PMCPMC13664919

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.