ArticleMolecules (Basel, Switzerland)2026
Purified and HPCCC Fractionated Anthocyanin-Rich Extracts from Black Bean Hulls Modulate H
Article in Molecules (Basel, Switzerland), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
This study aimed to obtain crude, purified, and fractionated anthocyanin-rich extracts of three black bean cultivars from the South Pacific Region of Mexico (Chiapas, Guerrero, and Oaxaca). The extracts were obtained using an Amberlite XAD-7HP column and high-performance countercurrent chromatography (HPCCC), and their modulatory effect on oxidative stress was evaluated in RAW 264.7 macrophages. Purification significantly increased the concentrations of phenolic compounds and anthocyanins across all cultivars. However, HPCCC fractionation produced a cultivar-dependent response, with marked anthocyanin enrichment observed only in the Guerrero cultivar. In contrast, a decrease was noted for Oaxaca and Chiapas, compared with the corresponding purified extracts. The anthocyanin content per gram of extract ranged from 12.1 to 21.4 mg/g for crude extracts, 27.4 to 85.5 mg/g for purified extracts, and 48 to 98.8 mg/g for fractionated extracts. The purification and fractionation processes enabled the obtaining of anthocyanin-rich extracts from black bean hulls that reduced H
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