ArticleMolecules (Basel, Switzerland)2026
Hemp Seed-Derived Vesicle-like Nanoparticles Modulate Dermal Collagen Homeostasis and Attenuate UVB-Induced Photoaging-Related Responses.
Article in Molecules (Basel, Switzerland), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Plant-derived extracellular vesicle-like nanoparticles (EVNPs) have emerged as bioactive nanomaterials with potential applications in skin biology; however, their isolation from complex plant matrices and their integrated biological effects remain insufficiently characterized. In this study, hemp seed-derived vesicle-like nanoparticles (hs- extracellular VLNPs) were isolated using a multi-step workflow combining pre-clarification, sequential filtration, electrokinetic capture, and tangential flow filtration. The isolated hs-VLNPs exhibited membrane-enclosed vesicle-like morphology, a nanoscale size distribution predominantly within 100-200 nm, and a negative surface charge. hs-VLNPs maintained cellular viability within the concentration range used for most functional assays and exhibited multiple biological activities associated with skin cellular homeostasis. In human dermal fibroblasts, hs-VLNPs increased procollagen type I production and attenuated UVB-induced matrix metalloproteinase-1 (MMP-1) expression following UVB exposure. In HaCaT keratinocytes, hs-VLNPs enhanced scratch closure under non-cytotoxic treatment conditions. Inflammatory mediator responses were cell- and mediator-dependent, with differential effects observed in RAW264.7 macrophages and human dermal fibroblasts rather than uniform suppression of inflammatory mediators. In addition, hs-VLNPs reduced α-melanocyte-stimulating hormone (α-MSH)-induced melanin production while exhibiting little direct inhibition of tyrosinase activity, suggesting that the anti-melanogenic effect may involve cellular regulatory mechanisms rather than direct enzymatic inhibition. Collectively, these findings demonstrate that electrokinetic capture combined with membrane-based filtration enables the enrichment of biologically active vesicle-like nanoparticles from hemp seeds. Rather than exerting a single biological effect, hs-VLNPs modulated multiple interconnected processes involving extracellular matrix homeostasis, cellular migration, immune responses, and melanogenesis, providing a foundation for further investigation of hemp seed-derived EVNPs as plant-derived nanomaterials for skin-related applications.
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