Development of an LC-MS/MS Method for Simultaneous Quantification of Erythromycin A and Seven Metabolites: Initial Residue Profile in Salmon Muscle with Skin.

Chaitali Chattopadhaya, Sean Knutson, Hernando Escobar Loaiza, Kenneth M Taylor, Charles Gieseker, Pak-Sin Chu

Abstract read

In one paragraph

Article in Molecules (Basel, Switzerland), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Chaitali ChattopadhayaDivision of Residue Chemistry, Office of Applied Science, Center for Veterinary Medicine, U.S. Food and Drug Administration (FDA), Laurel, MD 20708, USA.

Sean KnutsonDivision of Residue Chemistry, Office of Applied Science, Center for Veterinary Medicine, U.S. Food and Drug Administration (FDA), Laurel, MD 20708, USA.ORCID 0009-0006-4343-4863

Hernando Escobar LoaizaDivision of Residue Chemistry, Office of Applied Science, Center for Veterinary Medicine, U.S. Food and Drug Administration (FDA), Laurel, MD 20708, USA.

Kenneth M TaylorDivision of Residue Chemistry, Office of Applied Science, Center for Veterinary Medicine, U.S. Food and Drug Administration (FDA), Laurel, MD 20708, USA.ORCID 0000-0002-5130-5758

Charles GiesekerDivision of Residue Chemistry, Office of Applied Science, Center for Veterinary Medicine, U.S. Food and Drug Administration (FDA), Laurel, MD 20708, USA.

Pak-Sin ChuDivision of Residue Chemistry, Office of Applied Science, Center for Veterinary Medicine, U.S. Food and Drug Administration (FDA), Laurel, MD 20708, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A robust liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed and validated for determining erythromycin A (ERY A) and its metabolites: erythromycin B (ERY B), erythromycin C (ERY C), erythromycin A N-oxide (ENO), anhydroerythromycin A (AHEA), N-demethylerythromycin A (NDEA), erythromycin A enol ether (EAEE), and pseudoerythromycin A enol ether (PSEE) in salmon muscle with skin. Tissue samples (1.00 g) fortified with ERY A, ERY B, ERY C, ENO, EAEE, and PSEE at 50-200 ng/g and AHEA and NDEA at 500-2000 ng/g, using a surrogate internal standard, were extracted with acetonitrile, defatted hexane, and diluted water, resulting in accuracy from 87% to 108%, precision from 3% to 13%, and estimated LOQ from 0.02 to 1.2 ng/g. Incurred salmon showed ERY A as the major residue at 3 days post-administration and NDEA and AHEA as potential markers exceeding the 500 ng/g level on Day 28.

Indexed as

Drug ResiduesErythromycinLiquid Chromatography-Mass SpectrometryMusclesSalmonSkinTandem Mass SpectrometryAnimalsChromatography, LiquidErythromycinanalytical quantitationantibioticsAtlantic salmonerythromycin Aerythromycin metabolitesLC–MS/MSveterinary drug residues

Identifiers

PMID42883172

DOI10.3390/molecules31193406

PMCPMC13666175

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.