Evidence map›Paper›PMID 42883123›Full record

ArticleAnimals : an open access journal from MDPI2026

Visual and Equipment-Minimized Detection of Avian Metapneumovirus Subtype C by RT-RPA-CRISPR/Cas12a Coupled with Lateral Flow Dipstick.

Qizhang Liang, Shoukang Ma, Hongxing Li, Weiwei Wang, Qiuling Fu, Nansong Jiang, Rongchang Liu, Guanghua Fu, Hongmei Chen, Longfei Cheng and 2 more

Abstract read
In one paragraph

Article in Animals : an open access journal from MDPI, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Qizhang LiangFujian Academy of Agricultural Sciences/Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Institute of Animal Husbandry and Veterinary Medicine, Fuzhou 350013, China.
Shoukang MaCollege of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Hongxing LiCollege of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Weiwei WangFujian Academy of Agricultural Sciences/Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Institute of Animal Husbandry and Veterinary Medicine, Fuzhou 350013, China.ORCID 0000-0003-2336-6681
Qiuling FuFujian Academy of Agricultural Sciences/Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Institute of Animal Husbandry and Veterinary Medicine, Fuzhou 350013, China.ORCID 0000-0002-4005-8050
Nansong JiangFujian Academy of Agricultural Sciences/Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Institute of Animal Husbandry and Veterinary Medicine, Fuzhou 350013, China.
Rongchang LiuFujian Academy of Agricultural Sciences/Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Institute of Animal Husbandry and Veterinary Medicine, Fuzhou 350013, China.ORCID 0009-0000-7997-3275
Guanghua FuFujian Academy of Agricultural Sciences/Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Institute of Animal Husbandry and Veterinary Medicine, Fuzhou 350013, China.ORCID 0009-0004-9579-9691
Hongmei ChenFujian Academy of Agricultural Sciences/Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Institute of Animal Husbandry and Veterinary Medicine, Fuzhou 350013, China.
Longfei ChengFujian Academy of Agricultural Sciences/Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Institute of Animal Husbandry and Veterinary Medicine, Fuzhou 350013, China.
Chunhe WanFujian Academy of Agricultural Sciences/Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Institute of Animal Husbandry and Veterinary Medicine, Fuzhou 350013, China.ORCID 0000-0003-2011-5609
Yu HuangFujian Academy of Agricultural Sciences/Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Institute of Animal Husbandry and Veterinary Medicine, Fuzhou 350013, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Avian metapneumovirus (aMPV) is the main etiological agent of turkey rhinotracheitis, causing respiratory distress and economic losses in turkeys. While its impact in broilers and laying hens is relatively limited, aMPV subtype C (aMPV/C) is an important pathogen in ducks and waterfowl, leading to respiratory distress and severe egg-drop syndrome. To overcome the limitations of current diagnostics, such as heavy reliance on laboratory infrastructure and prolonged turnaround times, we established a rapid detection assay integrating recombinase polymerase amplification (RPA) with CRISPR/Cas12a and a lateral flow dipstick (LFD), targeting the conserved

Indexed as

avian metapneumovirusCRISPR/Cas12aLFDon-site detectionRPA

Identifiers

PMID42883123
PMCPMC13663432

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.