ArticleBioresources and bioprocessing2026
Integrating dynamic modeling and continuous cultivation to enhance recombinant β-glucosidase production in Komagataella phaffii.
Article in Bioresources and bioprocessing, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Cultivation under hypothermia has emerged as an interesting strategy for enhancing recombinant protein production in Komagataella phaffii, yet predictive approaches capable of integrating the effects of temperature and cell growth rate for rational bioprocess optimization remain limited. In this work, we investigated the influence of cultivation temperature on recombinant β-glucosidase (BGL1) production in the hybrid K. phaffii strain S467 by combining bioprocess characterization with dynamic mathematical modeling. Batch cultures performed at 20, 25 and 30 °C showed that hypothermia increased recombinant protein secretion while reducing biomass formation, revealing a trade-off between cell growth and product formation. To quantitatively describe these dynamics, a Droop-based model incorporating delayed coupling between substrate uptake and biomass synthesis was fitted using the experimental data. Model simulations predicted that continuous cultivation at 20 °C and a dilution rate of 0.045 h
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