Construction of an infectious clone of tomato leaf curl New Delhi virus and development of a duplex assay through recombinase polymerase amplification-lateral flow dipstick to detect the virus and tomato yellow leaf curl virus simultaneously.

Ning Qiao, Yuxing Chen, Yao Meng, Xiao Yang, Xin Wang, Lihao Zhou, Huijie Dai, Xiaoan Sun, Xiumei Ni

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Article in Frontiers in microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

9 authors.

Ning Qiao *Shandong Provincial University Laboratory of Green Diseases and Pests Management in Protected Vegetables, Weifang University of Science and Technology, Shouguang, Shandong, China.

Yuxing Chen *Shandong Provincial University Laboratory of Green Diseases and Pests Management in Protected Vegetables, Weifang University of Science and Technology, Shouguang, Shandong, China.

Yao Meng *Shandong Provincial University Laboratory of Green Diseases and Pests Management in Protected Vegetables, Weifang University of Science and Technology, Shouguang, Shandong, China.

Xiao YangShandong Provincial University Laboratory of Green Diseases and Pests Management in Protected Vegetables, Weifang University of Science and Technology, Shouguang, Shandong, China.

Xin WangShandong Provincial University Laboratory of Green Diseases and Pests Management in Protected Vegetables, Weifang University of Science and Technology, Shouguang, Shandong, China.

Lihao ZhouShandong Provincial University Laboratory of Green Diseases and Pests Management in Protected Vegetables, Weifang University of Science and Technology, Shouguang, Shandong, China.

Huijie DaiShandong Provincial University Laboratory of Green Diseases and Pests Management in Protected Vegetables, Weifang University of Science and Technology, Shouguang, Shandong, China.

Xiaoan SunShandong Provincial University Laboratory of Green Diseases and Pests Management in Protected Vegetables, Weifang University of Science and Technology, Shouguang, Shandong, China.

Xiumei NiShandong Provincial University Laboratory of Green Diseases and Pests Management in Protected Vegetables, Weifang University of Science and Technology, Shouguang, Shandong, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The co-infection of tomato yellow leaf curl virus (TYLCV) and tomato leaf curl New Delhi virus (ToLCNDV) in the same host complicates disease diagnosis and management, not to mention that each virus alone can cause devastating losses to its respective host crops across many tomato- and cucurbit-growing areas. However, diagnosis of mixed viral infections in the same host plants can be tedious, costly and challenging. This study aims to establish a rapid, sensitive, and specific duplex recombinase polymerase amplification-lateral flow dipstick (RPA-LFD) assay to simultaneously detect TYLCV and/or ToLCNDV in co-infected samples. We constructed an infectious clone from a ToLCNDV strain of the Asian clade isolated from a diseased cucumber plant in China, and confirmed that it could co-infect

Indexed as

infectious clonelateral flow dipstickrecombinase polymerase amplificationtomato leaf curl New Delhi virustomato yellow leaf curl virusviral co-infection

Identifiers

PMID42876742

DOI10.3389/fmicb.2026.1940758

PMCPMC13661962

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.