Evidence map›Paper›PMID 42875488›Full record

ArticleNature2026

De novo design of cysteine proteases.

Hojae Choi, Magnus S Bauer, Brian Coventry, Preetham Venkatesh, Anqi Chen, Donghyo Kim, Asim K Bera, Alex Kang, Hannah Nguyen, Shayan Sadre and 13 more

Abstract read
PubMed Publisher
In one paragraph

Article in Nature, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

23 authors.

Hojae ChoiDepartment of Biochemistry, University of Washington, Seattle, WA, USA.ORCID http://orcid.org/0009-0004-8035-1552
Magnus S BauerDepartment of Biochemistry, University of Washington, Seattle, WA, USA.ORCID http://orcid.org/0000-0003-1357-2852
Brian CoventryDepartment of Biochemistry, University of Washington, Seattle, WA, USA.ORCID http://orcid.org/0000-0002-6910-6255
Preetham VenkateshDepartment of Biochemistry, University of Washington, Seattle, WA, USA.
Anqi ChenDepartment of Biochemistry, University of Washington, Seattle, WA, USA.ORCID http://orcid.org/0000-0001-9857-0594
Donghyo KimDepartment of Biochemistry, University of Washington, Seattle, WA, USA.ORCID http://orcid.org/0000-0002-8097-3675
Asim K BeraDepartment of Biochemistry, University of Washington, Seattle, WA, USA.ORCID http://orcid.org/0000-0001-9473-2912
Alex KangDepartment of Biochemistry, University of Washington, Seattle, WA, USA.ORCID http://orcid.org/0000-0001-5487-0499
Hannah NguyenDepartment of Biochemistry, University of Washington, Seattle, WA, USA.ORCID http://orcid.org/0000-0001-9696-4004
Shayan SadreDepartment of Biochemistry, University of Washington, Seattle, WA, USA.
Justin DecarreauDepartment of Biochemistry, University of Washington, Seattle, WA, USA.
Emily JoyceDepartment of Biochemistry, University of Washington, Seattle, WA, USA.
Bhanumathi ShankaranMolecular Biophysics and Integrated Bioimaging, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.ORCID http://orcid.org/0000-0002-3266-8131
Tuscan Rock ThompsonDepartment of Biochemistry, University of Washington, Seattle, WA, USA.
Gabriel GreensteinInstitute for Protein Design, University of Washington, Seattle, WA, USA.ORCID http://orcid.org/0000-0001-7218-4708
Kieran DidiInstitute for Protein Design, University of Washington, Seattle, WA, USA.ORCID http://orcid.org/0000-0001-6839-3320
Lars L SchaafCavendish Laboratory, Department of Physics, University of Cambridge, Cambridge, UK.ORCID http://orcid.org/0000-0002-1935-236X
Jacob Merle GershonDepartment of Biochemistry, University of Washington, Seattle, WA, USA.
Alexander F ShidaDepartment of Biochemistry, University of Washington, Seattle, WA, USA.ORCID http://orcid.org/0000-0003-4140-6085
Gyu Rie LeeDepartment of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea.ORCID http://orcid.org/0000-0002-9119-5303
Donald HilvertLaboratory of Organic Chemistry, ETH Zurich, Zurich, Switzerland. hilvert@ethz.ch.ORCID http://orcid.org/0000-0002-3941-621X
Samuel J PellockDepartment of Biochemistry, University of Washington, Seattle, WA, USA. spellock@uw.edu.ORCID http://orcid.org/0000-0002-7557-7985
David BakerDepartment of Biochemistry, University of Washington, Seattle, WA, USA. dabaker@uw.edu.ORCID http://orcid.org/0000-0001-7896-6217

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Despite advances in de novo enzyme design, success has been largely limited to low-energy-barrier model reactions. Amide bonds such as those linking amino acids along the peptide backbone are stable for hundreds of years in neutral aqueous solution because of the high energy barrier to hydrolysis

Identifiers

PMID42875488

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.