Evidence map›Paper›PMID 42873646›Full record

ArticleSTAR protocols2026

Agarose-free protocol for preparing human organotypic lung cultures to study respiratory virus infection and evaluate antivirals ex vivo.

Lola Canus, Florentine Jacolin, Virginie Vasseur, Adeline Cezard, Eva Ogire, Anne Aublin-Gex, Amelie Bourgeais, Camille David, Alexandra Erny, Fabienne Archer and 8 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Lola CanusCIRI, Centre International de Recherche en Infectiologie, Team NeuroInvasion, Tropism and Viral Encephalitis, University Lyon, Inserm, U1111, CNRS, UMR5308, Université Lyon 1, Ecole Normale Supérieure de Lyon, 69007 Lyon, France.
Florentine JacolinCIRI, Centre International de Recherche en Infectiologie, Team Viral Infection, Metabolism and Immunity, University Lyon, Inserm, U1111, CNRS, UMR5308, Université Lyon 1, Ecole Normale Supérieure de Lyon, 69007 Lyon, France.
Virginie VasseurInserm, U1100, Centre D'Etude des Pathologies Respiratoires (CEPR), University of Tours, 37000 Tours, France.
Adeline CezardInserm, U1100, Centre D'Etude des Pathologies Respiratoires (CEPR), University of Tours, 37000 Tours, France.
Eva OgireCIRI, Centre International de Recherche en Infectiologie, Team NeuroInvasion, Tropism and Viral Encephalitis, University Lyon, Inserm, U1111, CNRS, UMR5308, Université Lyon 1, Ecole Normale Supérieure de Lyon, 69007 Lyon, France.
Anne Aublin-GexCIRI, Centre International de Recherche en Infectiologie, Team Viral Infection, Metabolism and Immunity, University Lyon, Inserm, U1111, CNRS, UMR5308, Université Lyon 1, Ecole Normale Supérieure de Lyon, 69007 Lyon, France.
Amelie BourgeaisCIRI, Centre International de Recherche en Infectiologie, Team NeuroInvasion, Tropism and Viral Encephalitis, University Lyon, Inserm, U1111, CNRS, UMR5308, Université Lyon 1, Ecole Normale Supérieure de Lyon, 69007 Lyon, France.
Camille DavidInserm, U1100, Centre D'Etude des Pathologies Respiratoires (CEPR), University of Tours, 37000 Tours, France.
Alexandra ErnyIVPC, Infections Virales et Pathologie Comparée, UMR754, INRAE, Université Lyon 1, EPHE, 69007 Lyon, France.
Fabienne ArcherIVPC, Infections Virales et Pathologie Comparée, UMR754, INRAE, Université Lyon 1, EPHE, 69007 Lyon, France.
Antoine LegrasService de Chirurgie Thoracique, CHRU de Tours, Université de Tours, Tours, France.
Damien SizaretService de Pathologie, CHRU de Tours, Tours, France.
Antoine GuillonInserm, U1100, Centre D'Etude des Pathologies Respiratoires (CEPR), University of Tours, 37000 Tours, France; Service de Médecine Intensive -Réanimation, CHRU de Tours, Tours, France.
Vincent LotteauLaboratoire P4 INSERM-Jean Mérieux, Lyon, France.
Pierre-Olivier VidalainCIRI, Centre International de Recherche en Infectiologie, Team Viral Infection, Metabolism and Immunity, University Lyon, Inserm, U1111, CNRS, UMR5308, Université Lyon 1, Ecole Normale Supérieure de Lyon, 69007 Lyon, France.
Mustapha Si-TaharInserm, U1100, Centre D'Etude des Pathologies Respiratoires (CEPR), University of Tours, 37000 Tours, France.
Laure Perrin-CoconCIRI, Centre International de Recherche en Infectiologie, Team Viral Infection, Metabolism and Immunity, University Lyon, Inserm, U1111, CNRS, UMR5308, Université Lyon 1, Ecole Normale Supérieure de Lyon, 69007 Lyon, France.
Cyrille MathieuCIRI, Centre International de Recherche en Infectiologie, Team NeuroInvasion, Tropism and Viral Encephalitis, University Lyon, Inserm, U1111, CNRS, UMR5308, Université Lyon 1, Ecole Normale Supérieure de Lyon, 69007 Lyon, France. Electronic address: cyrille.mathieu@inserm.fr.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Here, we present a protocol to generate human organotypic lung cultures from surgical lung resection for the study of respiratory infections. We describe steps for tissue harvesting, biopsy punching, mechanical slicing, and culture at the air-liquid interface. This technique enables robust ex vivo infections of human lung tissues with respiratory viruses, including influenza A and Nipah. It can be used to study host-pathogen interactions, analyze innate immune responses, and evaluate antiviral candidates in physiologically relevant human lung tissue. For complete details on the use and execution of this protocol, please refer to Cezard et al.

Indexed as

Cell cultureCell isolationHealth SciencesImmunologyMicrobiologyTissue Engineering

Identifiers

PMID42873646
PMCPMC13686045

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.