Evidence map›Paper›PMID 42872168›Full record

ArticleBioinformatics advances2026

BandQuant: a web application for western blot quantification.

Muyang He, Shanglin Jin, Hongjian Wan, Chengfeng Zhang

Abstract read
In one paragraph

Article in Bioinformatics advances, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Muyang HeDepartment of Dermatology, Huashan Hospital, Fudan University, Shanghai 200040, China.ORCID https://orcid.org/0009-0005-5595-4623
Shanglin JinDepartment of Dermatology, Huashan Hospital, Fudan University, Shanghai 200040, China.
Hongjian WanShanghai Art-Coding Software Co., Ltd., Shanghai 200052, China.
Chengfeng ZhangDepartment of Dermatology, Huashan Hospital, Fudan University, Shanghai 200040, China.ORCID https://orcid.org/0000-0002-1302-5667

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Motivation: Western blot quantification typically requires multiple tools and manual data handling, which is time-consuming and difficult to audit. Existing software is either tied to proprietary hardware or requires local installation, and none provides an integrated path from raw image to fold-change. Results: We present BandQuant, a free, browser-based web application that integrates the entire western blot quantification workflow into a single session. It combines automated band detection, rolling-ball background subtraction, housekeeping normalization, fold-change computation, and interactive bar-chart visualization in a single interface. A relational database binds experimental metadata to the image at upload, enabling automated normalization and cross-experiment queries without manual relabeling. BandQuant measurements showed high agreement with Fiji (Pearson Availability and implementation: BandQuant is freely available at https://www.tiaodaibao.com/? locale=en-US. Source code: https://github.com/wanhj-git/BandQuant. Archived release: https://zenodo.org/records/19453998.

Identifiers

PMID42872168
PMCPMC13661006

What OpenQuestion holds

Textmetadata
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.