Evidence map›Paper›PMID 42872074›Full record

ArticlePNAS nexus2026

A versatile fluorescent reporter for Usutu virus drug screening and infection tracking.

Amy N Nelson, Aaron E Lin, Saloni Sinha, Sydney J Mullin, Jianche Liu, Alexandra G Bell, Sebastian S Carver, Jason Sethiadi, Jiayu Zhang, Brigitte L Heller and 3 more

Abstract read
In one paragraph

Article in PNAS nexus, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Amy N NelsonDepartment of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.ORCID https://orcid.org/0000-0002-3654-163X
Aaron E LinDepartment of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.ORCID https://orcid.org/0000-0001-7400-4125
Saloni SinhaDivision of Gastroenterology and Hepatology, Department of Medicine, Weill Cornell Medicine, New York, NY 10065, USA.
Sydney J MullinDepartment of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.
Jianche LiuDepartment of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.ORCID https://orcid.org/0009-0000-8181-4206
Alexandra G BellDepartment of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.ORCID https://orcid.org/0009-0001-2036-0455
Sebastian S CarverDepartment of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.
Jason SethiadiDivision of Gastroenterology and Hepatology, Department of Medicine, Weill Cornell Medicine, New York, NY 10065, USA.
Jiayu ZhangDepartment of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.ORCID https://orcid.org/0009-0004-0955-7988
Brigitte L HellerDepartment of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.
Cameron MyhrvoldDepartment of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.ORCID https://orcid.org/0000-0002-8971-184X
Robert E SchwartzDivision of Gastroenterology and Hepatology, Department of Medicine, Weill Cornell Medicine, New York, NY 10065, USA.ORCID https://orcid.org/0000-0002-5417-5995
Alexander PlossDepartment of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.ORCID https://orcid.org/0000-0001-9322-7252

Funding

PREDOCTORAL TRAINING PROGRAM IN GENETICST32GM007388 · NIGMS · PRINCETON UNIVERSITY · PI CRISTEA, ILEANA M. · 1985 to 2022
$27.4M
Rational design and efficacy testing of vaccines against HCVR01AI168048 · NIAID · UNIV OF MARYLAND, COLLEGE PARK · PI Alexander Andrianov, Thomas R Fuerst · 2022 to 2026
$7.1M
Genetic Viral and Host Adaptations to Breach Species Barriers of HCVR01AI107301 · NIAID · PRINCETON UNIVERSITY · PI Thomas Pietschmann, Alexander Ploss · 2013 to 2026
$6.0M
Modeling immune impairments and pathogenesis in novel humanized mice for HBV-HIV co-infectionR01AI138797 · NIAID · UNIV OF NORTH CAROLINA CHAPEL HILL · PI PLOSS, ALEXANDER, SU, LISHAN · 2018 to 2022
$3.9M
Data-Driven Mathematical and Computational Modeling of Hepatitis D Infection and Treatment ResponseR01AI146917 · NIAID · LOYOLA UNIVERSITY CHICAGO · PI DAHARI, HAREL · 2020 to 2024
$3.5M
Targeting hepatitis B virus cccDNA during HBV/HIV co-infectionR01AI181664 · NIAID · PRINCETON UNIVERSITY · PI PLOSS, ALEXANDER · 2024 to 2025
$3.0M
Mechanisms of hepatitis B virus cccDNA formationR01AI153236 · NIAID · PRINCETON UNIVERSITY · PI PLOSS, ALEXANDER · 2020 to 2024
$2.8M
Optimization of the engineered 3D hepatic microenvironment enhances pluripotent stem cell derived hepatocyteR01DK121072 · NIDDK · WEILL MEDICAL COLL OF CORNELL UNIV · PI SCHWARTZ, ROBERT E · 2020 to 2024
$2.6M
Development of a highly-multiplexed CRISPR-based TB drug susceptibility testR01AI182281 · NIAID · RUTGERS BIOMEDICAL AND HEALTH SCIENCES · PI Cameron Amadeus Myhrvold, Yingda Linda Xie · 2024 to 2026
$2.2M
Modeling alcohol toxicity in human hepatocytesR01AA027327 · NIAAA · WEILL MEDICAL COLL OF CORNELL UNIV · PI DE JONG, YPE PETER · 2019 to 2023
$1.9M
BD FACSymphony A3: A multi-laser analytical flow cytometer for the Princeton University Flow Cytometry Resource FacilityS10OD028592 · OD · PRINCETON UNIVERSITY · PI DECOSTE, CHRISTINA J. · 2021 to 2021
$400k
Systematic dissection of viral genetic interactions and mutations with COEXPRES-SeqK99AI182471 · NIAID · PRINCETON UNIVERSITY · PI LIN, AARON ERIC · 2025 to 2025
$101k
NIAAA NIH HHS R01 AA027327NIAID NIH HHS K99 AI182471NIAID NIH HHS R01 AI107301NIAID NIH HHS R01 AI138797NIAID NIH HHS R01 AI146917NIAID NIH HHS R01 AI153236NIAID NIH HHS R01 AI168048NIAID NIH HHS R01 AI181664NIAID NIH HHS R01 AI182281NIDDK NIH HHS R01 DK121072NIGMS NIH HHS T32 GM007388NIH HHS S10 OD028592
6 · The paper itself

Abstract

Usutu virus (USUV) is an emerging mosquito-borne orthoflavivirus closely related to West Nile and Japanese encephalitis viruses. Despite its increasing prevalence in Europe and reports of neuroinvasive disease in humans, the molecular and immunological determinants of USUV infection remain poorly understood, largely due to the absence of tractable experimental tools. Here, we report the generation and characterization of the first full-length fluorescent reporter USUV. Using a previously established infectious clone of the Vienna 2001 strain, we inserted the

Indexed as

drug developmenthost responsesorthoflavivirusUsutu virusUSUV

Identifiers

PMID42872074
PMCPMC13659910

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.