ArticleFrontiers in plant science2026
Mixed seeding with sheep manure amendment alters soil nematode network complexity in a sandy grassland.
Article in Frontiers in plant science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Introduction: Soil nematodes are key bioindicators of terrestrial ecosystem functions, yet how restoration practices affect their community and interactions remains unclear. Methods: A one-year field experiment in an alpine grassland tested three treatments: bare sandy soil, mixed seeding of Result: High-throughput sequencing and co-occurrence network analysis revealed that mixed seeding with manure significantly enhanced plant biomass, soil organic carbon, electrical conductivity, and nematode richness (Chao1 index) by 26.36-31.58% ( Discussion: Both the mixed seeding treatment and the mixed seeding combined with manure treatment enhanced network complexity but increased competition over cooperation, reflecting altered interaction patterns. Whether this structural complexity translates into functional stability requires long-term monitoring. In conclusion, mixed seeding alone fosters a more structured soil food web with higher trophic-level representation, whereas the combination of mixed seeding with organic amendment-though boosting productivity and richness-induces salinization risks (elevated EC) and functional simplification toward bacterivore-dominated channels. The absence of a manure-only control limits our ability to attribute these effects solely to organic fertilization; future studies should include this treatment to isolate independent and interactive effects.
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