Evidence map›Paper›PMID 42870213›Full record

ArticleJournal of natural medicines2026

Development of a species-specific PCR-lateral flow assay integrated with SYBR Safe for rapid authentication of commercial Polygoni Multiflori Caulis (Ye-Jiao-Teng).

Ngoc-Phuoc Ngo, Yen-Ying Kung, PeiJung Chiang, Meng-Shiou Lee, Kai-Wei Chang, Kun-Chang Wu

Abstract read
PubMed Publisher
In one paragraph

Article in Journal of natural medicines, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Ngoc-Phuoc Ngo *School of Pharmacy, College of Pharmacy, China Medical University, No. 100, Section 1, Jingmao Road, Beitun District, Taichung, 406040, Taiwan.ORCID http://orcid.org/0009-0000-6829-6328
Yen-Ying Kung *Institute of Traditional Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan.
PeiJung Chiang *Department of Traditional Chinese Medicine, Taichung Veterans General Hospital, Taichung, Taiwan.
Meng-Shiou LeeDepartment of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, College of Chinese Medicine, China Medical University, Taichung, Taiwan.
Kai-Wei ChangInstitute of Traditional Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan.
Kun-Chang WuSchool of Pharmacy, College of Pharmacy, China Medical University, No. 100, Section 1, Jingmao Road, Beitun District, Taichung, 406040, Taiwan. kunchangwu@mail.cmu.edu.tw.ORCID http://orcid.org/0000-0001-5920-0064

Funding

China Medical University, Taiwan CMU110-MF-104China Medical University, Taiwan CMU111-N-16China Medical University, Taiwan CMU111-S-07China Medical University, Taiwan CMU112-S-54
6 · The paper itself

Abstract

Pleuropterus multiflorus (PM) is a widely used medicinal plant in traditional Chinese medicine (TCM). However, the closely related species Pleuropterus angulatus (PA) is commonly misidentified as PM due to morphological similarities, leading to frequent adulteration in commercial Polygoni Multiflori Caulis (PMC) products. To address this issue, we developed a species-specific PCR-lateral flow assay integrated with SYBR Safe for rapid and species-specific authentication of PM and PA. Species-specific primers were designed based on a diagnostic single nucleotide polymorphism (SNP) within the chloroplast trnL-trnF region. After validation of specificity, visual detection of PM and PA was achieved using lateral flow strips. The assay demonstrated detection limits of 0.5 ng/μL for PM and 0.05 ng/μL for PA genomic DNA. The application of this assay to eighteen commercial PMC products revealed the presence of PA in all samples, with only one product containing both PM and PA, indicating widespread adulteration in the herbal marketplace. In conclusion, the developed PCR-lateral flow assay provides a rapid, sensitive, and user-friendly molecular diagnostic tool suitable for authenticity testing of herbal products and quality control during pharmaceutical manufacturing.

Indexed as

Natural medicinesPleuropterus angulatusPleuropterus multiflorusPolygoni Multiflori CaulisPolymerase chain reaction-lateral flow assay (PCR-LFA)Quality control

Identifiers

PMID42870213

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.