Evidence map›Paper›PMID 42870082›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2027

Imaging Artificial Membranes Using High-Speed Atomic Force Microscopy.

Hussein Nasrallah, Anthony Vial, Nicolas Pocholle, Jérémy Soulier, Luca Costa, Cédric Godefroy, Eric Bourillot, Eric Lesniewska, Pierre-Emmanuel Milhiet

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2027. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Hussein NasrallahCentre de Biochimie Structurale (CBS), Univ Montpellier, INSERM, CNRS, Montpellier, 34090, France.
Anthony VialCentre de Biochimie Structurale (CBS), Univ Montpellier, INSERM, CNRS, Montpellier, 34090, France.
Nicolas PocholleICB UMR CNRS 6303, University of Bourgogne Franche-Comte, Dijon, F-21000, France.
Jérémy SoulierCentre de Biochimie Structurale (CBS), Univ Montpellier, INSERM, CNRS, Montpellier, 34090, France.
Luca CostaCentre de Biochimie Structurale (CBS), Univ Montpellier, INSERM, CNRS, Montpellier, 34090, France.
Cédric GodefroyCentre de Biochimie Structurale (CBS), Univ Montpellier, INSERM, CNRS, Montpellier, 34090, France.
Eric BourillotICB UMR CNRS 6303, University of Bourgogne Franche-Comte, Dijon, F-21000, France.
Eric LesniewskaICB UMR CNRS 6303, University of Bourgogne Franche-Comte, Dijon, F-21000, France.
Pierre-Emmanuel MilhietCentre de Biochimie Structurale (CBS), Univ Montpellier, INSERM, CNRS, Montpellier, 34090, France. pierre-emmanuel.milhiet@cnrs.fr.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Supported lipid bilayers represent a very attractive way to mimic biological membranes, especially to investigate molecular mechanisms associated with the lateral segregation of membrane components. Observation of these model membranes with high-speed atomic force microscopy (HS-AFM) allows the capture of both topography and dynamics of membrane components, with a spatial resolution in the nanometer range and image capture time of less than 1 s. In this context, we have developed new protocols adapted for HS-AFM to form supported lipid bilayers on small mica disks using the vesicle fusion or Langmuir-Blodgett methods. In this chapter, we describe in detail the protocols to fabricate supported artificial bilayers as well as the main guidelines for HS-AFM imaging of such samples.

Indexed as

Lipid BilayersMembranes, ArtificialMicroscopy, Atomic ForceAluminum SilicatesAluminum SilicatesLipid BilayersMembranes, ArtificialmicaArtificial membraneAtomic force microscopyDynamicsLangmuirLipidSupported lipid bilayerVesicle fusion

Identifiers

PMID42870082

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.