Evidence map›Paper›PMID 42867832›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Proximity Ligation Assay to Determine Protein-Protein Interactions in Muscle Stem Cells.

Marie J Catenacci, Michael A Rudnicki

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Marie J CatenacciSprott Centre for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, Canada.
Michael A RudnickiSprott Centre for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, Canada. mrudnicki@ohri.ca.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Satellite cells are present only in skeletal muscle and are crucial for regeneration. In recent years, satellite cell heterogeneity has identified different subpopulations, such as bona fide muscle stem cells. These populations are rare and in small numbers, making studying them very difficult. Moreover, satellite cells are sensitive to the environment and niche. To avoid niche disruption and changes to the cell state, our lab developed a mix of different techniques to study protein-protein interactions ex vivo on cultured myofibers. This protocol is based on the Proximity Ligation Assay (PLA) technique and uses IMARIS software to analyze protein interactions.

Indexed as

Muscle, SkeletalProtein Interaction MappingSatellite Cells, Skeletal MuscleStem CellsAnimalsCells, CulturedMiceMuscle Fibers, SkeletalNicheProtein complexProtein interactionSatellite cellsSkeletal muscle

Identifiers

PMID42867832

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.