Evidence map›Paper›PMID 42867827›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Flow Cytometric Methods for ROS Quantification in Skeletal Muscle Myotubes.

Andy Nolan, Paige Cole, Darren W Sexton

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Andy NolanResearch Institute for Sport and Exercise Sciences, Liverpool John Moores University, Liverpool, UK.
Paige ColeResearch Institute for Sport and Exercise Sciences, Liverpool John Moores University, Liverpool, UK.
Darren W SextonSchool of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Liverpool, UK. d.w.sexton@ljmu.ac.uk.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Flow cytometry is a high-throughput technique which can be used to analyze and quantify characteristics of individual cells. Flow cytometry hydrodynamically focuses cells past a series of size and fluorescent detectors to characterise cellular morphology and phenotype. This process of hydrodynamic focusing has previously been shown to be incompatible with myotubes due to their larger size relative to other cell types. Here, we describe the culture and differentiation of myotubes and how myotubes can be analyzed via flow cytometry to quantify changes in cellular and mitochondrial-specific ROS production.

Indexed as

Flow CytometryMuscle Fibers, SkeletalReactive Oxygen SpeciesAnimalsCell Culture TechniquesCell DifferentiationCell LineMiceMitochondriaReactive Oxygen SpeciesC2C12 myotubesFlow cytometryMitochondriaReactive oxygen speciesSkeletal muscle

Identifiers

PMID42867827

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.