Evidence map›Paper›PMID 42867826›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Extracellular Vesicle Isolation and Purification from Skeletal Muscle Cells.

Dongnan Yan, Emily Haughton, Ruhani Makkar, Matthew J A Wood, Dhanu Gupta

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Dongnan Yan *Institute of Developmental and Regenerative Medicine, Department of Paediatrics., University of Oxford, Oxford, UK.
Emily Haughton *Institute of Developmental and Regenerative Medicine, Department of Paediatrics., University of Oxford, Oxford, UK.
Ruhani MakkarInstitute of Developmental and Regenerative Medicine, Department of Paediatrics., University of Oxford, Oxford, UK.
Matthew J A WoodInstitute of Developmental and Regenerative Medicine, Department of Paediatrics., University of Oxford, Oxford, UK.
Dhanu GuptaInstitute of Developmental and Regenerative Medicine, Department of Paediatrics., University of Oxford, Oxford, UK. dhanu.gupta@paediatrics.ox.ac.uk.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Extracellular vesicles (EVs) have emerged as critical mediators of intercellular communication and hold considerable promise as biomarkers and therapeutic vectors. In skeletal muscle, EVs transport proteins, RNA, and other bioactive molecules, such as myokines, making them valuable indicators of muscle physiology and pathology. This method paper presents a detailed protocol for the isolation, purification, and characterization of EVs derived from human myoblasts. Our optimized workflow ensures high yield and sample quality, enabling comprehensive downstream molecular analysis. Additionally, we describe advanced techniques for assessing EV physical characteristics, concentration, and cargo content, providing an essential toolkit for investigating skeletal muscle EV biology and potential role in disease diagnostics. This protocol facilitates the efficient exploration of skeletal muscle-derived EVs and their implications in muscle function and pathology.

Indexed as

Cell FractionationExtracellular VesiclesMuscle, SkeletalHumansMyoblastsUltracentrifugationEV characterizationEV isolationEV purificationExtracellular vesiclesSkeletal muscleUltracentrifugation

Identifiers

PMID42867826

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.