Evidence map›Paper›PMID 42867823›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Analysis of Muscle Structure Using Fluorescently Tagged Proteins in Drosophila melanogaster Femur Muscles.

Tiara Mulder, Mayar Ibrabah, Francesca Di Cara, Nicanor González-Morales

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Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Tiara MulderDepartment of Biology, Life Sciences Centre, Dalhousie University, 6287 Alumni Crescent, Halifax, NS, Canada.
Mayar IbrabahDepartment of Biology, Life Sciences Centre, Dalhousie University, 6287 Alumni Crescent, Halifax, NS, Canada.
Francesca Di CaraDepartment of Microbiology and Immunology, Dalhousie University, Halifax, NS, Canada.
Nicanor González-MoralesDepartment of Biology, Life Sciences Centre, Dalhousie University, 6287 Alumni Crescent, Halifax, NS, Canada. nicanor.gonzalez@dal.ca.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The femur skeletal muscles of Drosophila melanogaster provide an excellent model for studying skeletal muscle development, function, physiology, and maintenance. Their key advantage over other adult skeletal muscles is the ability to image them with minimal dissection through the exoskeleton without compromising their structure, attachment sites, and overall morphology. Here, we present a general protocol for sample preparation and mounting of Drosophila legs that enables direct microscopy imaging of skeletal muscle structure. We provide representative images of the femur skeletal muscles under Apotome structured illumination microscopy and laser-scanning confocal microscopy. Finally, we highlight useful GFP or mCherry knock-in alleles of common skeletal muscle proteins that we have used successfully.

Indexed as

Drosophila melanogasterFemurMuscle, SkeletalAnimalsDrosophila ProteinsGreen Fluorescent ProteinsLuminescent ProteinsMicroscopy, ConfocalDrosophila ProteinsGreen Fluorescent ProteinsLuminescent ProteinsDrosophilaFemur musclesFluorescent proteinsLive imagingSarcomere structureSkeletal muscles

Identifiers

PMID42867823

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.