Evidence map›Paper›PMID 42867819›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Bulk Differentiation of Human Induced Pluripotent Stem Cells into Contractile Skeletal Muscles.

Yu-Shen Cheng, Farah Ulfat Jamee, Muhammad Irfanur Rashid, Hidetoshi Sakurai, Kazunori Shimizu, Yohei Okada

Abstract read
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Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Yu-Shen ChengDepartment of Neural iPSC Research, Institute for Medical Science of Aging, Aichi Medical University, 1-1 Yazakokarimata, Nagakute, Aichi, 480-1195, Japan.
Farah Ulfat JameeDepartment of Neural iPSC Research, Institute for Medical Science of Aging, Aichi Medical University, 1-1 Yazakokarimata, Nagakute, Aichi, 480-1195, Japan.
Muhammad Irfanur RashidDepartment of Neural iPSC Research, Institute for Medical Science of Aging, Aichi Medical University, 1-1 Yazakokarimata, Nagakute, Aichi, 480-1195, Japan.
Hidetoshi SakuraiDepartment of Clinical Application, Center for iPS Cell Research and Application (CiRA), Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto, 606-8507, Japan.
Kazunori ShimizuDepartment of Biomolecular Engineering, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8603, Japan.
Yohei OkadaDepartment of Neural iPSC Research, Institute for Medical Science of Aging, Aichi Medical University, 1-1 Yazakokarimata, Nagakute, Aichi, 480-1195, Japan. yohei@aichi-med-u.ac.jp.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Effective treatments for various muscular disorders and a clear understanding of their disease pathology remain lacking owing to the limitations of traditional animal models and two-dimensional (2D) cell cultures. Human induced pluripotent stem cells (hiPSCs) offer promising platforms for disease modeling and regenerative medicine. However, existing protocols for skeletal muscle differentiation are often labor-intensive and yield inconsistent results. Here, we utilized a piggyBac transposon-based system to generate bulk MYOD1-hiPSCs, eliminating the need for clonal selection and enabling rapid, efficient, and reproducible skeletal muscle differentiation. Furthermore, we established a protocol for generating functional three-dimensional (3D) skeletal muscle tissues that exert contractile force in response to electrical stimulation, highlighting their physiological relevance. This system provides a simple and valuable tool for disease modeling, drug screening, and the study of sporadic muscle disorders using patient-derived hiPSCs.

Indexed as

Cell Culture TechniquesCell DifferentiationInduced Pluripotent Stem CellsMuscle ContractionMuscle, SkeletalCells, CulturedHumansMuscle DevelopmentMyoD ProteinMyoD1 myogenic differentiation proteinMyoD Protein3D cultureBulk cultureContractionDisease modelingMYOD1-hiPSCSkeletal muscle differentiation

Identifiers

PMID42867819

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.