Isolation and Expansion of Muscle Satellite Cells Using the Ice-Cold Treatment Method.

Anna Benedetti, Lorenza Esposito, Marina Bouche, Biliana Lozanoska-Ochser

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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

4 authors.

Anna BenedettiDepartment of Anatomical, Histological, Forensic and Orthopedic Sciences, Section of Histology and Embryology, Sapienza University of Rome, Rome, Italy.

Lorenza EspositoDepartment of Anatomical, Histological, Forensic and Orthopedic Sciences, Section of Histology and Embryology, Sapienza University of Rome, Rome, Italy.

Marina BoucheDepartment of Anatomical, Histological, Forensic and Orthopedic Sciences, Section of Histology and Embryology, Sapienza University of Rome, Rome, Italy.

Biliana Lozanoska-OchserDepartment of Anatomical, Histological, Forensic and Orthopedic Sciences, Section of Histology and Embryology, Sapienza University of Rome, Rome, Italy. biliana.lozanoskaochser@unicamillus.org.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Satellite cells (SCs) are skeletal muscle stem cells responsible for muscle regeneration after injury. In this chapter, we describe the ice-cold treatment (ICT) method, a procedure developed for isolating and expanding highly pure populations of mouse and human SCs in vitro. The ICT method involves placing a dish containing a mixed population of adherent muscle mononuclear cells on ice for 15-30 min. This selectively causes only the SCs to detach, resulting in cell cultures with purity levels ranging from 95% to 100%. Furthermore, this method supports serial passaging, allowing prolonged expansion of SCs without negatively impacting their ability to proliferate or differentiate.

Indexed as

Cell Culture TechniquesCell SeparationSatellite Cells, Skeletal MuscleAnimalsCell DifferentiationCell ProliferationCells, CulturedCold TemperatureHumansIceMiceMuscle, SkeletalIceMyoDPax7Satellite cells in vitro expansionSatellite cells isolationSkeletal muscle

Identifiers

PMID42867816

DOI10.3390/ijms21030932

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.