Evidence map›Paper›PMID 42867815›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

An Optimized Pre-Plating Method for the Isolation and Culture of Human Primary Satellite Cells.

Guntarat Chinvattanachot, Naoki Horii, Tetsuro Masuda, Junki Kawakami, Takeshi Miyamoto, Yusuke Ono

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Guntarat ChinvattanachotDepartment of Muscle Development and Regeneration, Institute of Molecular Embryology and Genetics (IMEG), Kumamoto University, 2-2-1 Honjo, Chuo-ku, Kumamoto, 860-0811, Japan.
Naoki HoriiDepartment of Muscle Development and Regeneration, Institute of Molecular Embryology and Genetics (IMEG), Kumamoto University, 2-2-1 Honjo, Chuo-ku, Kumamoto, 860-0811, Japan.
Tetsuro MasudaDepartment of Orthopedic Surgery, Faculty of Life Sciences, Kumamoto University, 1-1-1 Honjo, Chuo-ku, Kumamoto, 860-8556, Japan.
Junki KawakamiDepartment of Orthopedic Surgery, Faculty of Life Sciences, Kumamoto University, 1-1-1 Honjo, Chuo-ku, Kumamoto, 860-8556, Japan.
Takeshi MiyamotoDepartment of Orthopedic Surgery, Faculty of Life Sciences, Kumamoto University, 1-1-1 Honjo, Chuo-ku, Kumamoto, 860-8556, Japan.
Yusuke OnoDepartment of Muscle Development and Regeneration, Institute of Molecular Embryology and Genetics (IMEG), Kumamoto University, 2-2-1 Honjo, Chuo-ku, Kumamoto, 860-0811, Japan. ono-y@kumamoto-u.ac.jp.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Muscle satellite cells play an essential role in postnatal skeletal muscle growth, homeostasis, hypertrophy, and regeneration following injury. Efficient primary culture methods are crucial for the in vitro analysis of satellite cells to investigate the molecular mechanisms underlying the adaptation and regeneration of skeletal muscles. In this protocol, we describe a refined and accessible pre-plating method for the efficient isolation of human satellite cells that does not require specialized equipment or advanced instrumentation. Furthermore, we provide comparative data on various growth medium formulations to identify the optimal and cost-effective conditions for human satellite cell culture. Through systematic optimization of both the isolation technique and culture conditions, we established a practical and reproducible protocol specifically tailored to human skeletal muscles. This methodology enables more accurate and clinically relevant investigations into human muscle biology.

Indexed as

Cell Culture TechniquesCell SeparationPrimary Cell CultureSatellite Cells, Skeletal MuscleCells, CulturedCulture MediaHumansMuscle, SkeletalCulture MediaHuman satellite cellsMuscle stem cellsPre-platingPrimary cultureSkeletal muscles

Identifiers

PMID42867815

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.