Evidence map›Paper›PMID 42867799›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2027

Construction of Library with Unique, Diverse DNA Barcodes in Salmonella Strains.

Ashley A Tarcin, Asim Zahoor Abbasi, Elena G Olson, Young Min Kwon, Steven C Ricke

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2027. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Ashley A Tarcin *Department of Animal and Dairy Sciences, University of Wisconsin, Madison, WI, USA.
Asim Zahoor Abbasi *Cell and Molecular Biology Program, University of Arkansas, Fayetteville, AR, USA.
Elena G OlsonDepartment of Animal and Dairy Sciences, University of Wisconsin, Madison, WI, USA.
Young Min KwonCell and Molecular Biology Program, University of Arkansas, Fayetteville, AR, USA.
Steven C RickeDepartment of Animal and Dairy Sciences, University of Wisconsin, Madison, WI, USA. sricke@wisc.edu.ORCID http://orcid.org/0000-0002-3584-9915

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This chapter details the methodology for generating DNA barcoded strains of Salmonella using a tri-parental conjugation. While this protocol was developed using S. typhimurium, it should remain applicable to additional Salmonella serotypes as well as other Gram-negative bacteria. The subsequently constructed library of DNA barcoded strains can be implemented, when complemented with next-generation sequencing or quantitative PCR, to quantitatively examine the impact of this pathogen on bacterial populations or transmission throughout a host.

Indexed as

DNA Barcoding, TaxonomicGene LibrarySalmonellaConjugation, GeneticDNA, BacterialDNA Transposable ElementsHigh-Throughput Nucleotide SequencingDNA, BacterialDNA Transposable ElementsDNA barcode-taggingNext generation sequencingPopulation dynamicsSalmonellaTn7 transposon systemTri-parental conjugation

Identifiers

PMID42867799

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.