Next-Day Salmonella spp. Detection Method Based on Real-Time PCR for Foods.

David Rodríguez-Lázaro, Mariana Alves, Álvaro Cañete-Reyes, Marta Hernández

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2027. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

David Rodríguez-LázaroMicrobiology Division, Faculty of Sciences, University of Burgos, Plaza Misael Bañuelos s/n, Burgos, Spain. drlazaro@ubu.es.ORCID http://orcid.org/0000-0002-8795-854X

Mariana AlvesMicrobiology Division, Faculty of Sciences, University of Burgos, Plaza Misael Bañuelos s/n, Burgos, Spain.ORCID http://orcid.org/0000-0003-2986-6900

Álvaro Cañete-ReyesMicrobiology Division, Faculty of Sciences, University of Burgos, Plaza Misael Bañuelos s/n, Burgos, Spain.ORCID http://orcid.org/0009-0003-9017-859X

Marta HernándezMicrobiology Division, Faculty of Medicine, University of Valladolid, Valladolid, Spain.ORCID http://orcid.org/0000-0001-6914-9987

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Reliable detection of Salmonella spp. remains essential for food safety, yet conventional culture-based methods are limited by their long turnaround times. Advances in molecular diagnostics, particularly real-time PCR (qPCR), have transformed pathogen detection by enabling faster, more sensitive, and more flexible analytical approaches. qPCR has gained prominence due to its ability to detect low levels of contamination and its robustness across diverse food matrices. In this chapter, we describe a qPCR-based strategy for the rapid detection of Salmonella spp. that adheres to the principles of ISO 6579-1:2017. The workflow integrates a short preenrichment step with a highly specific molecular assay, offering a streamlined and reliable alternative suited for modern food microbiology laboratories.

Indexed as

Food MicrobiologyReal-Time Polymerase Chain ReactionSalmonellaDNA, BacterialFood ContaminationDNA, BacterialFoodsRapid detectionReal-time PCRSalmonella spp.

Identifiers

PMID42867796

DOI10.1016/j.fm.2023.104299

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.