ArticleMethods in molecular biology (Clifton, N.J.)2027
Comet Assay for Genotoxicity Evaluation of Salmonella Outer Membrane Vesicles.
Article in Methods in molecular biology (Clifton, N.J.), 2027. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Authors and funding
4 authors.
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Abstract
Outer membrane vesicles (OMVs) from different Gram-negative bacteria, including Salmonella spp., have appeared as potential acellular vaccine candidates for their role in host-pathogen interaction by delivering virulence factors and modulating immune response. Given this host-cell contact, it is crucial to assess their genotoxic effects to ensure safety as vaccine candidates. The Comet assay, a sensitive and effective method for detecting single-cell DNA strand breaks, offers an effective approach for evaluating such effects. This study explores the application of the Comet assay as a highly sensitive approach to investigate the potential genotoxicity of Salmonella-derived OMVs in mammalian cells, with important implications for understanding host-pathogen interactions and cellular DNA damage, and providing a reproducible workflow for evaluating minimal DNA strand alterations in vitro as part of Salmonella-OMV safety studies.
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Registered trials
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