Evidence map›Paper›PMID 42867792›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2027

Generation and Use of Reporter Cell Lines to Analyze Integrity of Salmonella-Containing Vacuoles by Automated Live-Cell Imaging and Quantification.

Malte Kellermann, Michael Hensel

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2027. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Malte KellermannAbteilung Mikrobiologie and CellNanOs-Center of Cellular Nanoanalytics Osnabrück Fachbereich Biologie/Chemie, Universität Osnabrück, Osnabrück, Germany.ORCID http://orcid.org/0000-0001-8765-8275
Michael HenselAbteilung Mikrobiologie and CellNanOs-Center of Cellular Nanoanalytics Osnabrück Fachbereich Biologie/Chemie, Universität Osnabrück, Osnabrück, Germany. Michael.Hensel@uni-osnabrueck.de.ORCID http://orcid.org/0000-0001-6604-6253

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The stable expression of fluorescent reporters in cell lines is crucial for the analysis of host-pathogen interactions. Here, an optimized protocol for generating a HeLa cell line that indicates membrane damage, including lentiviral particle production, transduction, functional tests, and fluorescence-activated sorting (FACS) of HeLa cells, is described. In addition, an image-based segmentation protocol is described for quantifying membrane damage to Salmonella-containing vacuoles (SCV) and the presence of cytosolic Salmonella enterica. This enables the analysis of virulence factors that affect the integrity of SCV and thus contribute to the intracellular lifestyle of the pathogen.

Indexed as

Salmonella entericaSalmonella InfectionsVacuolesFlow CytometryGenes, ReporterHeLa CellsHost-Pathogen InteractionsHumansIntracellular pathogenLentiviral transductionLive-cell imagingMembrane integrity reporterPathogen-containing compartment

Identifiers

PMID42867792

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.