Evidence map›Paper›PMID 42867785›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2027

Generation of Random lacZY Transcriptional Fusions in the Genome of Salmonella enterica serovar Typhimurium.

Manuel A Cabral-Torres, Marta Bravo-García, Roberto Balbontín, Joaquín Bernal-Bayard, Francisco Ramos-Morales

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Article in Methods in molecular biology (Clifton, N.J.), 2027. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Manuel A Cabral-TorresDepartamento de Genética, Facultad de Biología, Universidad de Sevilla, Avda Reina Mercedes, Sevilla, Spain.
Marta Bravo-GarcíaDepartamento de Genética, Facultad de Biología, Universidad de Sevilla, Avda Reina Mercedes, Sevilla, Spain.
Roberto BalbontínDepartamento de Genética, Facultad de Biología, Universidad de Sevilla, Avda Reina Mercedes, Sevilla, Spain.
Joaquín Bernal-BayardDepartamento de Genética, Facultad de Biología, Universidad de Sevilla, Avda Reina Mercedes, Sevilla, Spain.
Francisco Ramos-MoralesDepartamento de Genética, Facultad de Biología, Universidad de Sevilla, Avda Reina Mercedes, Sevilla, Spain. framos@us.es.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Transcriptional fusions to the lacZ gene from Escherichia coli can be used to study gene expression. Here, we describe protocols for using a miniTn5 derivative to generate random insertions of the lacZY operon in the genome of Salmonella enterica serovar Typhimurium, to analyze the expression of transcriptional lac fusions under different conditions, and to identify the genomic location of some fusions.

Indexed as

Genome, BacterialLac OperonSalmonella typhimuriumTranscription, Geneticbeta-GalactosidaseEscherichia coliGene Expression Regulation, Bacterialbeta-GalactosidaselacZYminiTn5SalmonellaTranscriptional fusionβ-Galactosidase

Identifiers

PMID42867785

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.