Evidence map›Paper›PMID 42867780›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2027

Standardized Quantification of Salmonella Biofilms and Evaluation of Antibiotic Antibiofilm Activity.

Bibek Lamichhane, Yosra A Helmy

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2027. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Bibek LamichhaneDepartment of Veterinary Science, Martin-Gatton College of Agriculture, Food, and Environment, University of Kentucky, Lexington, KY, USA.ORCID http://orcid.org/0000-0002-1891-6266
Yosra A HelmyDepartment of Veterinary Science, Martin-Gatton College of Agriculture, Food, and Environment, University of Kentucky, Lexington, KY, USA. yosra.helmy@uky.edu.ORCID http://orcid.org/0000-0003-1470-5418

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Biofilm formation in Salmonella is a major factor contributing to its persistence and antimicrobial resistance. It contributes to both clinical and contamination in food-related environments, including food processing equipment, surfaces, and packaging materials. This chapter describes a standardized crystal violet (CV) microtiter plate assay for quantifying Salmonella biofilm formation and evaluating the potential antibiofilm effect of antibiotics (chloramphenicol) in inhibiting Salmonella biofilms, with detailed procedures and critical notes to help minimize common errors like over-washing or overstaining. Given the simplicity, reproducibility, and adaptability, this assay is a valuable tool for studying biofilm biology and evaluating antibiofilm strategies against Salmonella.

Indexed as

Anti-Bacterial AgentsBiofilmsSalmonellaChloramphenicolGentian VioletMicrobial Sensitivity TestsAnti-Bacterial AgentsChloramphenicolGentian VioletBiofilmsCrystal violetMicrotiter plate assaySalmonella

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.