ArticleVeterinary medicine and science2026
Detection of Mycobacterium avium subsp. paratuberculosis in Bovine Faeces Using a Rhodamine B Hydrazone Immunosensor on Antibody-Coated Nitrocellulose Membranes.
Article in Veterinary medicine and science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Rapid, inexpensive and reliable detection of Mycobacterium avium subsp. paratuberculosis (MAP) is essential for the control of Johne's disease. This study developed a rhodamine B hydrazone nanoparticle-based immunosensor coupled with antibody-coated nitrocellulose membranes (IMS-CNM) for the detection of MAP in bovine faecal samples. Anti-MAP antibodies were purified from hyperimmune donkey and rabbit sera. Purified donkey IgG was immobilized on nitrocellulose membranes as the capture antibody, whereas rabbit anti-MAP IgG was conjugated to rhodamine B hydrazone nanoparticles using EDC-NHS chemistry to prepare the chromogenic immunosensor. The diagnostic performance of the assay was evaluated using 89 bovine faecal samples and compared with nested PCR. Nested PCR identified 62 positive and 27 negative samples, whereas the IMS-CNM assay detected 60 positive and 29 negative samples. Using nested PCR as the reference method, the IMS-CNM assay achieved a sensitivity of 90.32% (95% CI: 82.96%-97.68%) and a specificity of 85.19% (95% CI: 71.80%-98.58%). The analytical limit of detection was approximately 3 × 10
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